V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody

Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2)

Synonyms: PKBB, PRKBB, PKBBETA, RAC-BETA, AKT2, cb945, prkbb, v-akt2, pkbbeta, rac-beta

Clonality: Monoclonal (1F8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393522

Additional Information

Alternative name: AKT2

Gene ID: 208

Immunogen: AKT2 (AAA58364, 100 a.a. ~ 189 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1F8

Description: Other names: PKBBETA, PRKBB, RACBETA v-akt murine thymoma viral oncogene homolog 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: AKT2 (AAA58364, 100 a.a. ~ 189 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 55769 DA

Comments:

Background: This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Phospho-specific antibodies, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

AKT2 monoclonal antibody (M04), clone 1F8 Western Blot analysis of AKT2 expression in Jurkat ( Cat # L017V1 ).

Publications

Product: Wickenden, Watson: "Key signalling nodes in mammary gland development and cancer. Signalling downstream of PI3 kinase in mammary epithelium: a play in 3 Akts." in: Breast cancer research : BCR, Vol. 12, Issue 2, pp. 202, 2010 (PubMed).

Foley, Bray, Tivnan et al.: "MicroRNA-184 inhibits neuroblastoma cell survival through targeting the serine/threonine kinase AKT2." in: Molecular cancer, Vol. 9, pp. 83, 2010 (PubMed).

Pu, Hildebrandt, Lu et al.: "PI3K/PTEN/AKT/mTOR pathway genetic variation predicts toxicity and distant progression in lung cancer patients receiving platinum-based chemotherapy." in: Lung cancer (Amsterdam, Netherlands), Vol. 71, Issue 1, pp. 82-8, 2010 (PubMed).

Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).