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Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) (AA 1-111) antibody

Details for Product No. ABIN393528, Supplier: Login to see
Antigen
  • Vhlh
  • HRCA1
  • RCA1
  • VHL1
  • pVHL
Epitope
AA 1-111
19
15
9
7
5
3
3
3
3
2
2
2
1
1
1
Reactivity
Human
101
45
40
4
1
1
Host
Mouse
62
23
16
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
71
50
31
17
11
8
8
5
2
2
1
1
1
Supplier
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Immunogen VHL (NP_000542, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1G12
Isotype IgG2b kappa
Specificity VHL (NP_000542, 1 a.a. ~ 111 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name VHL (VHL Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 24153 DA
Gene ID 12406
NCBI Accession NP_000542
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Transcription Factors, Cell Structure
Pathways
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) (AA 1-111) antibody (ABIN393528) Detection limit for recombinant GST tagged VHL is approximately 0.1 ng/mL as a captur...
Background publications Peña, Lathia, Shan et al.: "Biomarkers predicting outcome in patients with advanced renal cell carcinoma: Results from sorafenib phase III Treatment Approaches in Renal Cancer Global Evaluation Trial." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 19, pp. 4853-63, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Chacon-Camacho, Rodriguez-Dennen, Camacho-Molina et al.: "Clinical and molecular features of familial and sporadic cases of von Hippel-Lindau disease from Mexico." in: Clinical & experimental ophthalmology, Vol. 38, Issue 3, pp. 277-83, 2010 (PubMed).

Montani, Heinimann, von Teichman et al.: "VHL-gene deletion in single renal tubular epithelial cells and renal tubular cysts: further evidence for a cyst-dependent progression pathway of clear cell renal carcinoma in von Hippel-Lindau disease." in: The American journal of surgical pathology, Vol. 34, Issue 6, pp. 806-15, 2010 (PubMed).