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PCNA antibody (Proliferating Cell Nuclear Antigen) (AA 1-262)

Details for Product anti-PCNA Antibody No. ABIN393536, Supplier: Log in to see
Antigen
  • 53/13
  • CG9193
  • DmPCNA
  • Dmel\\CG9193
  • MUS209
  • Mus209
  • PCNA
  • PCNA/mus209
  • Pcna
  • dPCNA
  • l(2)02448
  • l(2)56F
  • l(2)56Fa
  • l(2)s1534
  • pcna
  • AO090003000846
  • PCNAR
  • Pcna/cyclin
  • cb16
  • fb36g03
  • wu:fa28e03
  • wu:fb36g03
  • pcna-A
Alternatives
anti-Human PCNA antibody for Immunohistochemistry
Epitope
AA 1-262
33
26
14
14
13
13
8
7
7
7
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
390
169
141
24
23
17
15
12
10
9
8
8
8
8
6
6
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
Host
Mouse
245
189
7
3
Clonality (Clone)
Monoclonal ()
Conjugate
This PCNA antibody is un-conjugated
16
14
7
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
314
146
113
110
83
73
69
30
28
23
7
7
6
3
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Immunogen PCNA (AAH00491, 1 a.a. ~ 262 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1G7
Isotype IgG2a kappa
Specificity PCNA (AAH00491, 1 a.a. ~ 262 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name PCNA (PCNA Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 28769 DA
Gene ID 12406
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Pathways Telomere Maintenance, DNA Damage Repair
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-PCNA antibody (Proliferating Cell Nuclear Antigen) (AA 1-262) (ABIN393536) Immunofluorescence of monoclonal antibody to PCNA on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-PCNA antibody (Proliferating Cell Nuclear Antigen) (AA 1-262) (ABIN393536) anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-262) antibody (Image 2)
Background publications Hervouet, Lalier, Debien et al.: "Disruption of Dnmt1/PCNA/UHRF1 interactions promotes tumorigenesis from human and mice glial cells." in: PLoS ONE, Vol. 5, Issue 6, pp. e11333, 2010 (PubMed).

Arora, Lindgren, Basu et al.: "Polymorphisms in the base excision repair pathway and graft-versus-host disease." in: Leukemia, Vol. 24, Issue 8, pp. 1470-5, 2010 (PubMed).

Locatelli, Pospiech, Tanguy Le Gac et al.: "Effect of 8-oxoguanine and abasic site DNA lesions on in vitro elongation by human DNA polymerase in the presence of replication protein A and proliferating-cell nuclear antigen." in: The Biochemical journal, Vol. 429, Issue 3, pp. 573-82, 2010 (PubMed).

Briggs, Goldstein, McCauley et al.: "Variation within DNA repair pathway genes and risk of multiple sclerosis." in: American journal of epidemiology, Vol. 172, Issue 2, pp. 217-24, 2010 (PubMed).

Monsees, Kraft, Chanock et al.: "Comprehensive screen of genetic variation in DNA repair pathway genes and postmenopausal breast cancer risk." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 207-14, 2010 (PubMed).