Proliferating Cell Nuclear Antigen (PCNA) (AA 1-262) antibody

Details for Product No. ABIN393536
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
53/13, CG9193, DmPCNA, Dmel\\CG9193, MUS209, Mus209, PCNA, PCNA/mus209, Pcna, dPCNA, l(2)02448, l(2)56F, l(2)56Fa, l(2)s1534, pcna, AO090003000846, PCNAR, Pcna/cyclin, cb16, fb36g03, wu:fa28e03, wu:fb ... show more
53/13, CG9193, DmPCNA, Dmel\\CG9193, MUS209, Mus209, PCNA, PCNA/mus209, Pcna, dPCNA, l(2)02448, l(2)56F, l(2)56Fa, l(2)s1534, pcna, AO090003000846, PCNAR, Pcna/cyclin, cb16, fb36g03, wu:fa28e03, wu:fb36g03, pcna-A show less
Epitope
AA 1-262
(24), (17), (15), (13), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(308), (180), (134), (79), (67), (62), (13), (13), (12), (12), (8), (7), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(182), (181), (4), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(20), (17), (6), (6), (6), (6), (6), (6), (6), (6), (6), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(246), (125), (76), (75), (61), (60), (53), (34), (24), (22), (4), (3), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
Shipping to United States (Change)
Availability Available
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN393536
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen PCNA (AAH00491, 1 a.a. ~ 262 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1G7
Isotype IgG2a kappa
Specificity PCNA (AAH00491, 1 a.a. ~ 262 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name PCNA
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 28769 DA
Gene ID 12406
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-262) antibody Immunofluorescence of monoclonal antibody to PCNA on HeLa cell (antibody concentration 10 µg/mL). PCNA monoclonal antibody (M02), clone 1G7 Western Blot analysis of PCNA expression in Hela.
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-262) antibody (2) anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-262) antibody (Image 2)
Background publications Monsees, Kraft, Chanock et al.: "Comprehensive screen of genetic variation in DNA repair pathway genes and postmenopausal breast cancer risk." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 207-14, 2010 (PubMed).

Briggs, Goldstein, McCauley et al.: "Variation within DNA repair pathway genes and risk of multiple sclerosis." in: American journal of epidemiology, Vol. 172, Issue 2, pp. 217-24, 2010 (PubMed).

Locatelli, Pospiech, Tanguy Le Gac et al.: "Effect of 8-oxoguanine and abasic site DNA lesions on in vitro elongation by human DNA polymerase in the presence of replication protein A and proliferating-cell nuclear antigen." in: The Biochemical journal, Vol. 429, Issue 3, pp. 573-82, 2010 (PubMed).

Arora, Lindgren, Basu et al.: "Polymorphisms in the base excision repair pathway and graft-versus-host disease." in: Leukemia, Vol. 24, Issue 8, pp. 1470-5, 2010 (PubMed).

Hervouet, Lalier, Debien et al.: "Disruption of Dnmt1/PCNA/UHRF1 interactions promotes tumorigenesis from human and mice glial cells." in: PLoS ONE, Vol. 5, Issue 6, pp. e11333, 2010 (PubMed).

Validation Images
Did you look for something else?
back to top