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Proliferating Cell Nuclear Antigen (PCNA) antibody

Antigen

Proliferating Cell Nuclear Antigen (PCNA)
Synonyms MGC8367, PCNAR, Pcna/cyclin, pcna
Clonality Monoclonal (1G7)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA, Immunofluorescence (IF), Western Blotting (WB)
5 references available
Certificates ISO 9001:2008
Catalog no. ABIN393536
Quantity 0.1mg
Price 450.00 $   Plus shipping costs $45.00
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anti-Proliferating Cell Nuclear Antigen (PCNA) antibody

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody (2)

Additional Information
Application Details Publications Images Alternatives back to top

Alternative name PCNA
Gene ID 5111
Immunogen PCNA (AAH00491, 1 a.a. ~ 262 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Isotype IgG2a kappa
Clone 1G7
Description Other names: MGC8367 proliferating cell nuclear antigen
Characteristics Purified Mouse Monoclonal Antibody (Mab)
Specificity PCNA (AAH00491, 1 a.a. ~ 262 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Molecular Weight 28769 DA
Comments

Background: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Application Details
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Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence
Purification Purified
Buffer Clear, colorless solution in phosphate buffered saline, pH 7.2 .
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Restrictions For Research Use only

Publications
Application Details Images Alternatives back to top

Product Monsees, Kraft, Chanock et al.: "Comprehensive screen of genetic variation in DNA repair pathway genes and postmenopausal breast cancer risk." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 207-14, 2010 (PubMed).

Briggs, Goldstein, McCauley et al.: "Variation within DNA repair pathway genes and risk of multiple sclerosis." in: American journal of epidemiology, Vol. 172, Issue 2, pp. 217-24, 2010 (PubMed).

Locatelli, Pospiech, Tanguy Le Gac et al.: "Effect of 8-oxoguanine and abasic site DNA lesions on in vitro elongation by human DNA polymerase in the presence of replication protein A and proliferating-cell nuclear antigen." in: The Biochemical journal, Vol. 429, Issue 3, pp. 573-82, 2010 (PubMed).

Arora, Lindgren, Basu et al.: "Polymorphisms in the base excision repair pathway and graft-versus-host disease." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 24, Issue 8, pp. 1470-5, 2010 (PubMed).

Hervouet, Lalier, Debien et al.: "Disruption of Dnmt1/PCNA/UHRF1 interactions promotes tumorigenesis from human and mice glial cells." in: PLoS ONE, Vol. 5, Issue 6, pp. e11333, 2010 (PubMed).

Alternatives
Application Details Publications Images back to top

Alternatives for antigen "Proliferating Cell Nuclear Antigen (PCNA)", type "Antibodies"
Hosts Rabbit (162), Mouse (133), Goat (3), Rat (2)
Reactivities Human (259), Rat (Rattus) (178), Mouse (Murine) (151), Cow (Bovine) (119), Chicken (95), Dog (Canine) (95), Sheep (Ovine) (36), Pig (Porcine) (23), Guinea Pig (18), Insect (7), Hamster (6), Vertebrates (5), Rabbit (4), Yeast (3), Yeast (Saccharomyces Cerevisiae) (3), Algae (2), Fish (2), Mammalian (2), Microalgae (Isochrysis galbana) (2), Monkey (2), Xenopus laevis (2), Amphibian (1), Cat (Feline) (1), Fruit Fly (Drosophila melanogaster) (1), Yeast (Pichia pastoris) (1), Zebrafish (1)
Applications Western Blotting (WB) (162), Immunofluorescence (IF) (113), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (94), ELISA (71), Immunoprecipitation (IP) (49), Immunohistochemistry (IHC) (44), Flow Cytometry (FACS) (35), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (15), Immunocytochemistry (ICC) (13), Immunohistochemistry (Fixed) (IHC (fx)) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunoelectron Microscopy (IEM) (6), Cellular Assay (CA) (1), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunostaining (ISt) (1)
Conjugates FITC (17), Biotin (15), PE (9), Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), Cy3 (6), Cy5 (6), Cy5.5 (6), Cy7 (6), Gold (6), HRP (6), PE,Cy3 (6), PE,Cy5 (6), PE,Cy5.5 (6), PE,Cy7 (6), APC (1), RPE (1)
Epitopes C-Term (68), N-Term (18), pTyr211 (18), Internal Region (6), AA 55-71 (4), AA 1-261 (3), Center (2), AA 111-112 (1), AA 111-122 (1), AA 75-125 (1), C-Term,ubLys164 (1), Internal Region,AA 111-122 (1), Nuclear (1), Tyr211 (1), pGlu1,pThr395 (1)