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TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) (AA 1784-1893) antibody

Details for Product No. ABIN393538
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Antigen
Synonyms
BG:DS00004.13, CG17603, Dmel\\CG17603, EfW1, SR3-5, TAF, TAF1, TAF200, TAF230, TAF250, TAF250/230, TAFII-250, TAFII250, TAF[II]250, TAF[[II]], TAF[[II]]230, TAF[[II]]250, TAF[[II]]250/230, TFIID, TFII ... show more
BG:DS00004.13, CG17603, Dmel\\CG17603, EfW1, SR3-5, TAF, TAF1, TAF200, TAF230, TAF250, TAF250/230, TAFII-250, TAFII250, TAF[II]250, TAF[[II]], TAF[[II]]230, TAF[[II]]250, TAF[[II]]250/230, TFIID, TFIID TAF250, Taf1p, Taf200, Taf230, Taf250, Taf[[II]]250, cel, cell, d230, dTAF230, dTAF250, dTAFII250, dTAF[[II]]230, dTAF[[II]]250, dmTAF1, dmTAF[[II]]230, l(3)84Ab, p230, 250kDa, AU015687, B430306D02Rik, Ccg-1, Ccg1, KAT4, N-TAF1, Taf2a, RGD1562050, BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, NSCL2, OF, P250, TAF2A, XDP, TAF(II)250, p250 show less
Epitope
AA 1784-1893
(14), (8), (5), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(35), (26), (3), (2)
Host
Mouse
(39), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(32), (26), (11), (8), (2), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN393538
450.00 $
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Immunogen TAF1 (NP_004597, 1784 a.a. ~ 1893 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1G9
Isotype IgG Mix kappa
Specificity TAF1 (NP_004597, 1784 a.a. ~ 1893 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name TAF1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 212677 DA
Gene ID 12406
UniProt NP_004597
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. This gene is part of a complex transcriptional unit (TAF1/DYT3), wherein some products share exons with TAF1 as well as additional exons downstream.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1) (AA 1784-1893) antibody TAF1 monoclonal antibody (M02), clone 1G9 Western Blot analysis of TAF1 expression in Hela NE.
Background publications Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Olejnik-Schmidt, Schmidt, Kedzia et al.: "Search for cellular partners of human papillomavirus type 16 E2 protein." in: Archives of virology, Vol. 153, Issue 5, pp. 983-90, 2008 (PubMed).

Allende-Vega, McKenzie, Meek: "Transcription factor TAFII250 phosphorylates the acidic domain of Mdm2 through recruitment of protein kinase CK2." in: Molecular and cellular biochemistry, Vol. 316, Issue 1-2, pp. 99-106, 2008 (PubMed).

Centeno, Ramírez-Salazar, García-Villa et al.: "TAF1 interacts with and modulates human papillomavirus 16 E2-dependent transcriptional regulation." in: Intervirology, Vol. 51, Issue 2, pp. 137-43, 2008 (PubMed).

Tavassoli, Wafa, Cheng et al.: "TAF1 differentially enhances androgen receptor transcriptional activity via its N-terminal kinase and ubiquitin-activating and -conjugating domains." in: Molecular endocrinology (Baltimore, Md.), Vol. 24, Issue 4, pp. 696-708, 2010 (PubMed).

Validation Images
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