TGF-beta Activated Kinase 1/MAP3K7 Binding Protein 1 (TAB1) antibody

Antigen: TGF-beta Activated Kinase 1/MAP3K7 Binding Protein 1 (TAB1)

Synonyms: TAB1, 3'-Tab1, MGC57664

Clonality: Monoclonal (2A12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393549

Additional Information

Alternative name: MAP3K7IP1

Gene ID: 10454

UniProt: NP_705717

Immunogen: MAP3K7IP1 (NP_705717, 3 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2A12

Description: Other names: MGC57664, TAB1mitogen-activated protein kinase kinase kinase 7 interacting protein 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: MAP3K7IP1 (NP_705717, 3 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 54644 DA

Comments:

Background: The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 0.41mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to MAP3K7IP1 (AT2780a) on HeLa cell . [antibody concentration 10 ug/ml] MAP3K7IP1 monoclonal antibody (M03), clone 2A12 Western Blot analysis of MAP3K7IP1 expression in HeLa ( Cat # L013V1 ).

anti-TGF-beta Activated Kinase 1/MAP3K7 Binding Protein 1 (TAB1) antibody (Image 2)

Publications

Product: Isono, Kim, Ando et al.: "Suppression of cell invasiveness by periostin via TAB1/TAK1." in: International journal of oncology, Vol. 35, Issue 2, pp. 425-32, 2009 (PubMed).

Crawford, Yang, Mattaini et al.: "The metastasis efficiency modifier ribosomal RNA processing 1 homolog B (RRP1B) is a chromatin-associated factor." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28660-73, 2009 (PubMed).

Potter, Cordell, Barton et al.: "Association between anti-tumour necrosis factor treatment response and genetic variants within the TLR and NF{kappa}B signalling pathways." in: Annals of the rheumatic diseases, Vol. 69, Issue 7, pp. 1315-20, 2010 (PubMed).

Scholz, Sidler, Thali et al.: "Autoactivation of transforming growth factor beta-activated kinase 1 is a sequential bimolecular process." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25753-66, 2010 (PubMed).

McGovern, Jones, Taylor et al.: "Fucosyltransferase 2 (FUT2) non-secretor status is associated with Crohn's disease." in: Human molecular genetics, Vol. 19, Issue 17, pp. 3468-76, 2010 (PubMed).