Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1 (MAPKSP1) (AA 1-88) antibody

Details for Product No. ABIN393554
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Antigen
Synonyms MAPKSP1, MAP2K1IP1, MAPBP, MP1, PRO0633, Ragulator3, AW556229, Map2k1ip1, Mapbp, Mapksp1, Mp1, mapksp1, zgc:110207
Epitope
AA 1-88
(5), (5), (4), (2), (1)
Reactivity
Human
(29), (20), (15), (12), (12)
Host
Mouse
(26), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(20), (11), (10), (7), (6), (5), (3), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393554
450.00 $
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Immunogen MAP2K1IP1 (NP_068805, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2A4
Isotype IgG1 kappa
Specificity MAP2K1IP1 (NP_068805, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MAP2K1IP1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 13623 DA
Gene ID 12406
NCBI Accession NP_068805
Research Area Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a scaffold protein that functions in the extracellular signal-regulated kinase (ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2. Studies of the orthologous gene in mouse indicate that it regulates late endosomal traffic and cell proliferation. Multiple transcript variants are expressed by this gene, but only one variant is thought to express a protein.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1 (MAPKSP1) (AA 1-88) antibody Western Blot analysis of MAP2K1IP1 expression in transfected 293T cell line by MAP2K1IP1 monoclonal antibody (M03), clone 2A4. Lane 1: MAP2K1IP1 transfected lysate(13.623 KDa). Lane 2: Non-transfected lysate.
Background publications Villegas, Njaine, Linden et al.: "Glial-derived neurotrophic factor (GDNF) prevents ethanol (EtOH) induced B92 glial cell death by both PI3K/AKT and MEK/ERK signaling pathways." in: Brain research bulletin, Vol. 71, Issue 1-3, pp. 116-26, 2006 (PubMed).

Teis, Taub, Kurzbauer et al.: "p14-MP1-MEK1 signaling regulates endosomal traffic and cellular proliferation during tissue homeostasis." in: The Journal of cell biology, Vol. 175, Issue 6, pp. 861-8, 2006 (PubMed).

Brahma, Dalby: "Regulation of protein phosphorylation within the MKK1-ERK2 complex by MP1 and the MP1*P14 heterodimer." in: Archives of biochemistry and biophysics, Vol. 460, Issue 1, pp. 85-91, 2007 (PubMed).

Vomastek, Iwanicki, Schaeffer et al.: "RACK1 targets the extracellular signal-regulated kinase/mitogen-activated protein kinase pathway to link integrin engagement with focal adhesion disassembly and cell motility." in: Molecular and cellular biology, Vol. 27, Issue 23, pp. 8296-305, 2007 (PubMed).

Sancak, Bar-Peled, Zoncu et al.: "Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids." in: Cell, Vol. 141, Issue 2, pp. 290-303, 2010 (PubMed).

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