Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1 (MAPKSP1) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1 (MAPKSP1)

Synonyms: MP1, MAPBP, MAP2K1IP1, MGC106009, MAPKSP1

Clonality: Monoclonal (2A4)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393554

Additional Information

Alternative name: MAP2K1IP1

Gene ID: 8649

UniProt: NP_068805

Immunogen: MAP2K1IP1 (NP_068805, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2A4

Description: Other names: MP1 mitogen-activated protein kinase kinase 1 interacting protein 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: MAP2K1IP1 (NP_068805, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 13623 DA

Comments:

Background: This gene encodes a scaffold protein that functions in the extracellular signal-regulated kinase (ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2. Studies of the orthologous gene in mouse indicate that it regulates late endosomal traffic and cell proliferation. Multiple transcript variants are expressed by this gene, but only one variant is thought to express a protein.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of MAP2K1IP1 expression in transfected 293T cell line by MAP2K1IP1 monoclonal antibody (M03), clone 2A4. Lane 1: MAP2K1IP1 transfected lysate(13.623 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Villegas, Njaine, Linden et al.: "Glial-derived neurotrophic factor (GDNF) prevents ethanol (EtOH) induced B92 glial cell death by both PI3K/AKT and MEK/ERK signaling pathways." in: Brain research bulletin, Vol. 71, Issue 1-3, pp. 116-26, 2006 (PubMed).

Teis, Taub, Kurzbauer et al.: "p14-MP1-MEK1 signaling regulates endosomal traffic and cellular proliferation during tissue homeostasis." in: The Journal of cell biology, Vol. 175, Issue 6, pp. 861-8, 2006 (PubMed).

Brahma, Dalby: "Regulation of protein phosphorylation within the MKK1-ERK2 complex by MP1 and the MP1*P14 heterodimer." in: Archives of biochemistry and biophysics, Vol. 460, Issue 1, pp. 85-91, 2007 (PubMed).

Vomastek, Iwanicki, Schaeffer et al.: "RACK1 targets the extracellular signal-regulated kinase/mitogen-activated protein kinase pathway to link integrin engagement with focal adhesion disassembly and cell motility." in: Molecular and cellular biology, Vol. 27, Issue 23, pp. 8296-305, 2007 (PubMed).

Sancak, Bar-Peled, Zoncu et al.: "Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids." in: Cell, Vol. 141, Issue 2, pp. 290-303, 2010 (PubMed).