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Methyl-CpG Binding Domain Protein 1 (MBD1) (AA 415-509) antibody

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Synonyms CXXC3, PCM1, RFT, Cxxc3
AA 415-509
(13), (6), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
(74), (20), (16), (5), (2)
(56), (20)
Clonality (Clone)
Monoclonal ()
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(62), (16), (11), (8), (6), (5), (2), (2), (2), (1)
Pubmed 5 references available
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Quantity 0.1 mg
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Availability Will be delivered in 8 to 11 Business Days
Immunogen MBD1 (NP_056671, 415 a.a. ~ 509 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2B10
Isotype IgG2b kappa
Specificity MBD1 (NP_056671, 415 a.a. ~ 509 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MBD1 (MBD1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 66607 DA
Gene ID 12406
NCBI Accession NP_056671
Research Area Signaling, Protein Modifications, Transcription Factors, Cell Structure
Application Notes Western blot = 1:500-1000

Background: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Methyl-CpG Binding Domain Protein 1 (MBD1) (AA 415-509) antibody (ABIN393559) MBD1 monoclonal antibody (M05), clone 2B10. Western Blot analysis of MBD1 expression ...
Background publications Crowther-Swanepoel, Broderick, Di Bernardo et al.: "Common variants at 2q37.3, 8q24.21, 15q21.3 and 16q24.1 influence chronic lymphocytic leukemia risk." in: Nature genetics, Vol. 42, Issue 2, pp. 132-6, 2010 (PubMed).

Bierne, Tham, Batsche et al.: "Human BAHD1 promotes heterochromatic gene silencing." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 33, pp. 13826-31, 2009 (PubMed).

Liu, Jin, Wang et al.: "Methyl-CpG binding domain 1 gene polymorphisms and lung cancer risk in a Chinese population." in: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, Vol. 13, Issue 6, pp. 607-17, 2008 (PubMed).

Yaqinuddin, Abbas, Naqvi et al.: "Silencing of MBD1 and MeCP2 in prostate-cancer-derived PC3 cells produces differential gene expression profiles and cellular phenotypes." in: Bioscience reports, Vol. 28, Issue 6, pp. 319-26, 2008 (PubMed).

Liu, Chen, Yu et al.: "Proteomic analysis of differential proteins in pancreatic carcinomas: Effects of MBD1 knock-down by stable RNA interference." in: BMC cancer, Vol. 8, pp. 121, 2008 (PubMed).

Catalog No. ABIN393559
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