|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Methyl-CpG Binding Domain Protein 1 (MBD1) antibody
|Synonyms||RFT, PCM1, CXXC3|
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||MBD1 (NP_056671, 415 a.a. ~ 509 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: CXXC3, PCM1, RFT methyl-CpG binding domain protein 1|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||MBD1 (NP_056671, 415 a.a. ~ 509 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||66607 DA|
Background: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Signaling, Protein Modifications, Transcription Factors, Cell Structure|
|Restrictions||For Research Use only|
|MBD1 monoclonal antibody (M05), clone 2B10. Western Blot analysis of MBD1 expression in Jurkat ( Cat # L017V1 ).|
Liu, Chen, Yu et al.: "Proteomic analysis of differential proteins in pancreatic carcinomas: Effects of MBD1 knock-down by stable RNA interference." in: BMC cancer, Vol. 8, pp. 121, 2008 (PubMed).
Yaqinuddin, Abbas, Naqvi et al.: "Silencing of MBD1 and MeCP2 in prostate-cancer-derived PC3 cells produces differential gene expression profiles and cellular phenotypes." in: Bioscience reports, Vol. 28, Issue 6, pp. 319-26, 2008 (PubMed).
Liu, Jin, Wang et al.: "Methyl-CpG binding domain 1 gene polymorphisms and lung cancer risk in a Chinese population." in: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, Vol. 13, Issue 6, pp. 607-17, 2008 (PubMed).
Bierne, Tham, Batsche et al.: "Human BAHD1 promotes heterochromatic gene silencing." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 33, pp. 13826-31, 2009 (PubMed).
Crowther-Swanepoel, Broderick, Di Bernardo et al.: "Common variants at 2q37.3, 8q24.21, 15q21.3 and 16q24.1 influence chronic lymphocytic leukemia risk." in: Nature genetics, Vol. 42, Issue 2, pp. 132-6, 2010 (PubMed).
|Hosts||Rabbit (41), Mouse (5)|
|Reactivities||Human (43), Mouse (Murine) (23), Rat (Rattus) (21), Cow (Bovine) (2), Dog (Canine) (2)|
|Applications||Western Blotting (WB) (31), Immunofluorescence (IF) (15), ELISA (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (7), N-Term (3), AA 33-69 (1), AA 59-4 (1), AA 593-605 (1), AA 98-113 (1), Internal Region (1)|