Methyl-CpG Binding Domain Protein 1 (MBD1) antibody

Antigen: Methyl-CpG Binding Domain Protein 1 (MBD1)

Synonyms: RFT, PCM1, CXXC3

Clonality: Monoclonal (2B10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393559

Additional Information

Alternative name: MBD1

Gene ID: 4152

UniProt: NP_056671

Immunogen: MBD1 (NP_056671, 415 a.a. ~ 509 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 2B10

Description: Other names: CXXC3, PCM1, RFT methyl-CpG binding domain protein 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: MBD1 (NP_056671, 415 a.a. ~ 509 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 66607 DA

Comments:

Background: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

MBD1 monoclonal antibody (M05), clone 2B10. Western Blot analysis of MBD1 expression in Jurkat ( Cat # L017V1 ).

Publications

Product: Liu, Chen, Yu et al.: "Proteomic analysis of differential proteins in pancreatic carcinomas: Effects of MBD1 knock-down by stable RNA interference." in: BMC cancer, Vol. 8, pp. 121, 2008 (PubMed).

Yaqinuddin, Abbas, Naqvi et al.: "Silencing of MBD1 and MeCP2 in prostate-cancer-derived PC3 cells produces differential gene expression profiles and cellular phenotypes." in: Bioscience reports, Vol. 28, Issue 6, pp. 319-26, 2008 (PubMed).

Liu, Jin, Wang et al.: "Methyl-CpG binding domain 1 gene polymorphisms and lung cancer risk in a Chinese population." in: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, Vol. 13, Issue 6, pp. 607-17, 2008 (PubMed).

Bierne, Tham, Batsche et al.: "Human BAHD1 promotes heterochromatic gene silencing." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 33, pp. 13826-31, 2009 (PubMed).

Crowther-Swanepoel, Broderick, Di Bernardo et al.: "Common variants at 2q37.3, 8q24.21, 15q21.3 and 16q24.1 influence chronic lymphocytic leukemia risk." in: Nature genetics, Vol. 42, Issue 2, pp. 132-6, 2010 (PubMed).