Aldehyde Dehydrogenase 18 Family, Member A1 (ALDH18A1) antibody

Antigen: Aldehyde Dehydrogenase 18 Family, Member A1 (ALDH18A1)

Synonyms: GSAS, P5CS, PYCS, MGC117316, Pycs, AI429789, MGC32233, 2810433K04Rik, ALDH18A1, MGC127306, pycs, cb842, cb899, sb:cb881, MGC162616, wu:fa91f10, wu:fi05f11, wu:fi17d12, DKFZp459G021, MGC81784

Clonality: Monoclonal (2B5)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393567

Additional Information

Alternative name: ALDH18A1

Gene ID: 5832

UniProt: NP_002851

Immunogen: ALDH18A1 (NP_002851, 696 a.a. ~ 796 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 2B5

Description: Other names: GSAS, MGC117316, P5CS, PYCS aldehyde dehydrogenase 18 family, member A1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ALDH18A1 (NP_002851, 696 a.a. ~ 796 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 87302 DA

Comments:

Background: This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine. Mutations in this gene lead to hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia and may be associated with neurodegeneration, cataracts and connective tissue diseases. Alternatively spliced transcript variants, encoding different isoforms, have been described for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

ALDH18A1 monoclonal antibody (M01), clone 2B5 Western Blot analysis of ALDH18A1 expression in MCF-7 ( Cat # L046V1 ).

Publications

Product: Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Hu, Khalil, Zhaorigetu et al.: "Human Delta1-pyrroline-5-carboxylate synthase: function and regulation." in: Amino acids, Vol. 35, Issue 4, pp. 665-72, 2008 (PubMed).

Bicknell, Pitt, Aftimos et al.: "A missense mutation in ALDH18A1, encoding Delta1-pyrroline-5-carboxylate synthase (P5CS), causes an autosomal recessive neurocutaneous syndrome." in: European journal of human genetics : EJHG, Vol. 16, Issue 10, pp. 1176-86, 2008 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).