Transducin (Beta)-Like 1X-Linked (TBL1X) antibody

Antigen: Transducin (Beta)-Like 1X-Linked (TBL1X)

Synonyms: EBI, TBL1, SMAP55, TBL1X, tbl1x, MGC158242

Clonality: Monoclonal (2B6)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN393572

Additional Information

Alternative name: TBL1X

Gene ID: 6907

UniProt: NP_005638

Immunogen: TBL1X (NP_005638, 478 a.a. ~ 578 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 2B6

Description: Other names: EBI, TBL1 transducin (beta)-like 1X-linked

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: TBL1X (NP_005638, 478 a.a. ~ 578 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 57049 DA

Comments:

Background: The protein encoded by this gene has sequence similarity with members of the WD40 repeat-containing protein family. The WD40 group is a large family of proteins, which appear to have a regulatory function. It is believed that the WD40 repeats mediate protein-protein interactions and members of the family are involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation. This encoded protein is found as a subunit in corepressor SMRT (silencing mediator for retinoid and thyroid receptors) complex along with histone deacetylase 3 protein. This gene is located adjacent to the ocular albinism gene and it is thought to be involved in the pathogenesis of the ocular albinism with late-onset sensorineural deafness phenotype. Four transcript variants encoding two different isoforms have been found for this gene. This gene is highly similar to the Y chromosome TBL1Y gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Cell Signaling, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Immunoperoxidase of monoclonal antibody to TBL1X ( Cat# AT4162a ) on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 1.5 ug/ml] Detection limit for recombinant GST tagged TBL1X is approximately 0.3ng/ml as a capture antibody.

anti-Transducin (Beta)-Like 1X-Linked (TBL1X) antibody (Image 2)

Publications

Product: Yoon, Choi, Cole et al.: "Reading and function of a histone code involved in targeting corepressor complexes for repression." in: Molecular and cellular biology, Vol. 25, Issue 1, pp. 324-35, 2004 (PubMed).

Gerlitz, Darhin, Giorgio et al.: "Novel functional features of the Lis-H domain: role in protein dimerization, half-life and cellular localization." in: Cell cycle (Georgetown, Tex.), Vol. 4, Issue 11, pp. 1632-40, 2005 (PubMed).

Li, Wang: "TBL1-TBLR1 and beta-catenin recruit each other to Wnt target-gene promoter for transcription activation and oncogenesis." in: Nature cell biology, Vol. 10, Issue 2, pp. 160-9, 2008 (PubMed).

Ishii, Kurasawa, Wong et al.: "Histone deacetylase 3 localizes to the mitotic spindle and is required for kinetochore-microtubule attachment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 11, pp. 4179-84, 2008 (PubMed).

Perissi, Scafoglio, Zhang et al.: "TBL1 and TBLR1 phosphorylation on regulated gene promoters overcomes dual CtBP and NCoR/SMRT transcriptional repression checkpoints." in: Molecular cell, Vol. 29, Issue 6, pp. 755-66, 2008 (PubMed).