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Hect Domain and RLD 3 (HERC3) (AA 1-110) antibody

Details for Product No. ABIN393576, Supplier: Log in to see
Antigen
  • 5730409F18Rik
  • AI646603
  • mKIAA0032
Epitope
AA 1-110
17
17
5
5
4
3
1
1
1
Reactivity
Human
51
3
2
Host
Mouse
41
10
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
38
25
11
4
2
Supplier
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Immunogen HERC3 (NP_055421, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C1
Isotype IgG1 kappa
Specificity HERC3 (NP_055421, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name HERC3 (HERC3 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 117188 DA
Gene ID 12406
NCBI Accession NP_055421
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: HERC3 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Hect Domain and RLD 3 (HERC3) (AA 1-110) antibody (ABIN393576) Immunoperoxidase of monoclonal antibody to HERC3 on formalin-fixed paraffin-embedded ...
Immunohistochemistry (IHC) image for anti-Hect Domain and RLD 3 (HERC3) (AA 1-110) antibody (ABIN393576) anti-Hect Domain and RLD 3 (HERC3) (AA 1-110) antibody (Image 2)
Background publications Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Hochrainer, Kroismayr, Baranyi et al.: "Highly homologous HERC proteins localize to endosomes and exhibit specific interactions with hPLIC and Nm23B." in: Cellular and molecular life sciences : CMLS, Vol. 65, Issue 13, pp. 2105-17, 2008 (PubMed).

Hochrainer, Mayer, Baranyi et al.: "The human HERC family of ubiquitin ligases: novel members, genomic organization, expression profiling, and evolutionary aspects." in: Genomics, Vol. 85, Issue 2, pp. 153-64, 2005 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Cruz, Ventura, Bartrons et al.: "HERC3 binding to and regulation by ubiquitin." in: FEBS letters, Vol. 488, Issue 1-2, pp. 74-80, 2001 (PubMed).