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Hect Domain and RLD 3 (HERC3) antibody

Details for Product No. ABIN393576
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Antigen
Synonyms 5730409F18Rik, AI646603, mKIAA0032
Reactivity
Human
(40), (1), (1)
Host
Mouse
(26), (14)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(30), (23), (10), (6), (4), (3), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN393576
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen HERC3 (NP_055421, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C1
Isotype IgG1 kappa
Specificity HERC3 (NP_055421, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name HERC3
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 117188 DA
Gene ID 12406
UniProt NP_055421
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: HERC3 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Hect Domain and RLD 3 (HERC3) antibody Immunoperoxidase of monoclonal antibody to HERC3 on formalin-fixed paraffin-embedded human pancreas (antibody concentration 3 µg/mL). Detection limit for recombinant GST tagged HERC3 is approximately 0.1 ng/mL as a capture antibody.
anti-Hect Domain and RLD 3 (HERC3) antibody (2) anti-Hect Domain and RLD 3 (HERC3) antibody (Image 2)
Background publications Cruz, Ventura, Bartrons et al.: "HERC3 binding to and regulation by ubiquitin." in: FEBS letters, Vol. 488, Issue 1-2, pp. 74-80, 2001 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Hochrainer, Mayer, Baranyi et al.: "The human HERC family of ubiquitin ligases: novel members, genomic organization, expression profiling, and evolutionary aspects." in: Genomics, Vol. 85, Issue 2, pp. 153-64, 2005 (PubMed).

Hochrainer, Kroismayr, Baranyi et al.: "Highly homologous HERC proteins localize to endosomes and exhibit specific interactions with hPLIC and Nm23B." in: Cellular and molecular life sciences : CMLS, Vol. 65, Issue 13, pp. 2105-17, 2008 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Hosts (26), (14)
Reactivities (40), (1), (1)
Applications (30), (23), (10), (6), (4), (3), (1)
Conjugates (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (9), (8), (6), (5), (2), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN393576
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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