Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) antibody

Antigen: Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS)

Synonyms: MACS, 80K-L, PKCSL, PRKCSL, FLJ14368, FLJ90045, KINC, Macs, marcks, MGC89127, MARCKS, MGC137530

Clonality: Monoclonal (2C2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393581

Additional Information

Alternative name: MARCKS

Gene ID: 4082

UniProt: NP_002347

Immunogen: MARCKS (NP_002347, 2 a.a. ~ 66 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2C2

Description: Other names: 80K-L, FLJ14368, MACS, MRACKS, PKCSL, PRKCSL myristoylated alanine-rich protein kinase C substrate

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: MARCKS (NP_002347, 2 a.a. ~ 66 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 31555 DA

Comments:

Background: The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to MARCKS ( Cat# AT2803a ) on HeLa cell . [antibody concentration 10 ug/ml] MARCKS monoclonal antibody (M06), clone 2C2. Western Blot analysis of MARCKS expression in Raw 264.7. (OMIN,80 kDa,http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=177061

anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) antibody (Image 2)

Publications

Product: Eckert, Neuder, Park et al.: "Myristoylated alanine-rich C-kinase substrate (MARCKS) protein regulation of human neutrophil migration." in: American journal of respiratory cell and molecular biology, Vol. 42, Issue 5, pp. 586-94, 2010 (PubMed).

Micallef, Taccone, Mukherjee et al.: "Epidermal growth factor receptor variant III-induced glioma invasion is mediated through myristoylated alanine-rich protein kinase C substrate overexpression." in: Cancer research, Vol. 69, Issue 19, pp. 7548-56, 2009 (PubMed).

Finlayson, Freeman: "A cell motility screen reveals role for MARCKS-related protein in adherens junction formation and tumorigenesis." in: PLoS ONE, Vol. 4, Issue 11, pp. e7833, 2009 (PubMed).

Techasen, Loilome, Namwat et al.: "Myristoylated alanine-rich C kinase substrate phosphorylation promotes cholangiocarcinoma cell migration and metastasis via the protein kinase C-dependent pathway." in: Cancer science, Vol. 101, Issue 3, pp. 658-65, 2010 (PubMed).

Lin, Fang, Park et al.: "MARCKS and related chaperones bind to unconventional myosin V isoforms in airway epithelial cells." in: American journal of respiratory cell and molecular biology, Vol. 43, Issue 2, pp. 131-6, 2010 (PubMed).