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Details for Product No. ABIN393581

Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) antibody

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Antigen
Synonyms 80K-L, MACS, PKCSL, PRKCSL, Macs, KINC, macs, marcks, wu:fb55c06, wu:fb92d04, wu:fc19b04, wu:fc44a10, zgc:110235, MGC89127, MARCKS
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN393581
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen MARCKS (NP_002347, 2 a.a. ~ 66 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C2
Isotype IgG1 kappa
Specificity MARCKS (NP_002347, 2 a.a. ~ 66 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MARCKS
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 31555 DA
Gene ID 12406
UniProt NP_002347
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence
Comment

Background: The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Eckert, Neuder, Park et al.: "Myristoylated alanine-rich C-kinase substrate (MARCKS) protein regulation of human neutrophil migration." in: American journal of respiratory cell and molecular biology, Vol. 42, Issue 5, pp. 586-94, 2010 (PubMed).

Micallef, Taccone, Mukherjee et al.: "Epidermal growth factor receptor variant III-induced glioma invasion is mediated through myristoylated alanine-rich protein kinase C substrate overexpression." in: Cancer research, Vol. 69, Issue 19, pp. 7548-56, 2009 (PubMed).

Finlayson, Freeman: "A cell motility screen reveals role for MARCKS-related protein in adherens junction formation and tumorigenesis." in: PLoS ONE, Vol. 4, Issue 11, pp. e7833, 2009 (PubMed).

Techasen, Loilome, Namwat et al.: "Myristoylated alanine-rich C kinase substrate phosphorylation promotes cholangiocarcinoma cell migration and metastasis via the protein kinase C-dependent pathway." in: Cancer science, Vol. 101, Issue 3, pp. 658-65, 2010 (PubMed).

Lin, Fang, Park et al.: "MARCKS and related chaperones bind to unconventional myosin V isoforms in airway epithelial cells." in: American journal of respiratory cell and molecular biology, Vol. 43, Issue 2, pp. 131-6, 2010 (PubMed).

Alternatives for antigen "Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS)", type "Antibodies"
Hosts (189), (9), (2)
Reactivities (176), (134), (125), (53), (40), (36), (24), (4), (3), (1), (1), (1)
Applications (139), (53), (47), (40), (29), (21), (16), (5), (4), (2), (1), (1)
Conjugates (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Epitopes (28), (26), (23), (22), (21), (9), (7), (6), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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