Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (AA 1-95) antibody

Details for Product No. ABIN393588
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Antigen
Synonyms e2(17)kb1, sft, ubc4/5, ubch5, ubch5a, E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A, ube2d1, wu:fc16h06, zgc:73096
Epitope
AA 1-95
(10), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(39), (20), (17), (13), (2), (1), (1), (1), (1)
Host
Mouse
(36), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(30), (16), (13), (10), (9), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393588
450.00 $
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Immunogen UBE2D1 (NP_003329, 1 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C6
Isotype IgG2a kappa
Specificity UBE2D1 (NP_003329, 1 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2D1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 16602 DA
Gene ID 12406
NCBI Accession NP_003329
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Transcription Factors, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (AA 1-95) antibody Immunoperoxidase of monoclonal antibody to UBE2D1 on formalin-fixed paraffin-embedded human cervix cancer (antibody concentration 1 µg/mL). Detection limit for recombinant GST tagged UBE2D1 is approximately 0.3 ng/mL as a capture antibody.
anti-Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (AA 1-95) antibody (2) anti-Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (AA 1-95) antibody (Image 2)
Background publications Markson, Kiel, Hyde et al.: "Analysis of the human E2 ubiquitin conjugating enzyme protein interaction network." in: Genome research, Vol. 19, Issue 10, pp. 1905-11, 2009 (PubMed).

van Wijk, de Vries, Kemmeren et al.: "A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system." in: Molecular systems biology, Vol. 5, pp. 295, 2009 (PubMed).

Zeng, Xu, Liu et al.: "Key role of Ubc5 and lysine-63 polyubiquitination in viral activation of IRF3." in: Molecular cell, Vol. 36, Issue 2, pp. 315-25, 2009 (PubMed).

Graf, Stankiewicz, Nikolay et al.: "Insights into the conformational dynamics of the E3 ubiquitin ligase CHIP in complex with chaperones and E2 enzymes." in: Biochemistry, Vol. 49, Issue 10, pp. 2121-9, 2010 (PubMed).

Wu, Kovacev, Pan: "Priming and extending: a UbcH5/Cdc34 E2 handoff mechanism for polyubiquitination on a SCF substrate." in: Molecular cell, Vol. 37, Issue 6, pp. 784-96, 2010 (PubMed).

Validation Images
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