Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN393588

Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms SFT, UBCH5, UBC4/5, UBCH5A, E2(17)KB1, ube2d1, wu:fc16h06, zgc:73096, sft, ubch5, ubc4/5, ubch5a, e2(17)kb1
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN393588
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 2 to 3 Business Days
Immunogen UBE2D1 (NP_003329, 1 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C6
Isotype IgG2a kappa
Specificity UBE2D1 (NP_003329, 1 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2D1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 16602 DA
Gene ID 12406
UniProt NP_003329
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Transcription Factors, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Markson, Kiel, Hyde et al.: "Analysis of the human E2 ubiquitin conjugating enzyme protein interaction network." in: Genome research, Vol. 19, Issue 10, pp. 1905-11, 2009 (PubMed).

van Wijk, de Vries, Kemmeren et al.: "A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system." in: Molecular systems biology, Vol. 5, pp. 295, 2009 (PubMed).

Zeng, Xu, Liu et al.: "Key role of Ubc5 and lysine-63 polyubiquitination in viral activation of IRF3." in: Molecular cell, Vol. 36, Issue 2, pp. 315-25, 2009 (PubMed).

Graf, Stankiewicz, Nikolay et al.: "Insights into the conformational dynamics of the E3 ubiquitin ligase CHIP in complex with chaperones and E2 enzymes." in: Biochemistry, Vol. 49, Issue 10, pp. 2121-9, 2010 (PubMed).

Wu, Kovacev, Pan: "Priming and extending: a UbcH5/Cdc34 E2 handoff mechanism for polyubiquitination on a SCF substrate." in: Molecular cell, Vol. 37, Issue 6, pp. 784-96, 2010 (PubMed).

Alternatives for antigen "Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1)", type "Antibodies"
Hosts (27), (3)
Reactivities (29), (19), (15), (13), (1), (1), (1), (1), (1)
Applications (21), (10), (10), (8), (7), (2)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (2), (2), (1), (1)
Beacon