Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) antibody

Details for Product No. ABIN393588
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Antigen
Synonyms SFT, UBCH5, UBC4/5, UBCH5A, E2(17)KB1, ube2d1, wu:fc16h06, zgc:73096, sft, ubch5, ubc4/5, ubch5a, e2(17)kb1
Reactivity
Human
(29), (19), (16), (13), (1), (1), (1), (1), (1)
Host
Mouse
(27), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(21), (10), (10), (8), (8), (1)
Pubmed 5 references available
Catalog no. ABIN393588
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen UBE2D1 (NP_003329, 1 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C6
Isotype IgG2a kappa
Specificity UBE2D1 (NP_003329, 1 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2D1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 16602 DA
Gene ID 12406
UniProt NP_003329
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Transcription Factors, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) antibody Immunoperoxidase of monoclonal antibody to UBE2D1 on formalin-fixed paraffin-embedded human cervix cancer (antibody concentration 1 µg/mL). Detection limit for recombinant GST tagged UBE2D1 is approximately 0.3 ng/mL as a capture antibody.
anti-Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) antibody (2) anti-Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) antibody (Image 2)
Background publications Markson, Kiel, Hyde et al.: "Analysis of the human E2 ubiquitin conjugating enzyme protein interaction network." in: Genome research, Vol. 19, Issue 10, pp. 1905-11, 2009 (PubMed).

van Wijk, de Vries, Kemmeren et al.: "A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system." in: Molecular systems biology, Vol. 5, pp. 295, 2009 (PubMed).

Zeng, Xu, Liu et al.: "Key role of Ubc5 and lysine-63 polyubiquitination in viral activation of IRF3." in: Molecular cell, Vol. 36, Issue 2, pp. 315-25, 2009 (PubMed).

Graf, Stankiewicz, Nikolay et al.: "Insights into the conformational dynamics of the E3 ubiquitin ligase CHIP in complex with chaperones and E2 enzymes." in: Biochemistry, Vol. 49, Issue 10, pp. 2121-9, 2010 (PubMed).

Wu, Kovacev, Pan: "Priming and extending: a UbcH5/Cdc34 E2 handoff mechanism for polyubiquitination on a SCF substrate." in: Molecular cell, Vol. 37, Issue 6, pp. 784-96, 2010 (PubMed).

Hosts (27), (3)
Reactivities (29), (19), (16), (13), (1), (1), (1), (1), (1)
Applications (21), (10), (10), (8), (8), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (2), (2), (1), (1)
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