Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibody

Details for Product No. ABIN393594
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Antigen
Synonyms 7420452D20Rik, ASK, ASK1, MAPKKK5, Mekk5, mitogen-activated protein kinase kinase kinase 5, MUD21.11, MUD21_11, MEKK5, MAP3K5
Epitope
AA 1231-1374
(48), (41), (33), (16), (13), (12), (12), (12), (10), (7), (6), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(317), (129), (77), (36), (24), (12), (1)
Host
Mouse
(301), (18)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(10), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4)
Application
Immunofluorescence (IF), Western Blotting (WB)
(233), (119), (113), (64), (60), (34), (31), (8), (5), (4), (3), (3), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN393594
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Immunogen MAP3K5 (AAH54503, 1231 a.a. ~ 1374 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2D11
Isotype IgG1 lambda
Specificity MAP3K5 (AAH54503, 1231 a.a. ~ 1374 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MAP3K5
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 154537 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibody Immunofluorescence of monoclonal antibody to MAP3K5 on A-431 cell (antibody concentration 10 µg/mL). Western Blot analysis of MAP3K5 expression in transfected 293T cell line by MAP3K5 monoclonal antibody (M03), clone 2D11. Lane 1: MAP3K5 transfected lysate(155 KDa). Lane 2: Non-transfected lysate.
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibody (2) anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibody (Image 2)
Background publications Mo, Jung, Yoon et al.: "DJ-1 modulates the p38 mitogen-activated protein kinase pathway through physical interaction with apoptosis signal-regulating kinase 1." in: Journal of cellular biochemistry, Vol. 110, Issue 1, pp. 229-37, 2010 (PubMed).

Sturchler, Feurstein, McDonald et al.: "Mechanism of oxidative stress-induced ASK1-catalyzed MKK6 phosphorylation." in: Biochemistry, Vol. 49, Issue 19, pp. 4094-102, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Yang, Yu, Guan et al.: "ASK1-JNK signaling cascade mediates Ad-ST13-induced apoptosis in colorectal HCT116 cells." in: Journal of cellular biochemistry, Vol. 110, Issue 3, pp. 581-8, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Validation Images
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