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MGC10540 (AA 1-177) antibody

Details for Product No. ABIN393603
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Antigen
Epitope
AA 1-177
Reactivity
Human
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Immunofluorescence (IF), Western Blotting (WB)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393603
450.00 $
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Immunogen MGC10540 (AAH06282, 1 a.a. ~ 177 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2E5-2B9
Isotype IgG1 kappa
Specificity MGC10540 (AAH06282, 1 a.a. ~ 177 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 20748 DA
Gene ID 12406
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL, MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741], Kamura et al., 2001 [PubMed 11278625]).

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-MGC10540 (AA 1-177) antibody Immunofluorescence of monoclonal antibody to VPS25 on HeLa cell (antibody concentration 10 µg/mL). MGC10540 monoclonal antibody (M01), clone 2E5-2B9 Western Blot analysis of MGC10540 expression in Hela NE.
anti-MGC10540 (AA 1-177) antibody (2) anti-MGC10540 (AA 1-177) antibody (Image 2)
Background publications Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Bowers, Piper, Edeling et al.: "Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII." in: The Journal of biological chemistry, Vol. 281, Issue 8, pp. 5094-105, 2006 (PubMed).

Im, Wollert, Boura et al.: "Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis." in: Developmental cell, Vol. 17, Issue 2, pp. 234-43, 2009 (PubMed).

Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Validation Images
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