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Details for Product No. ABIN393616

Ubiquitin Protein Ligase E3A (ube3a) antibody

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Antigen
Synonyms
ANCR, AS, E6-AP, EPVE6AP, HPVE6A, im:7140733, zgc:92173, 4732496B02, 5830462N02Rik, A130086L21Rik, Hpve6a, mKIAA4216, As, CG6190, Dmel\\CG6190, Dube3a, UBE3A, dUBE3A, das, dube3A, dube3a, UME3A, DDBDR ... show more
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN393616
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen UBE3A (AAH09271, 51 a.a. ~ 151 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2F6
Isotype IgG2a kappa
Specificity UBE3A (AAH09271, 51 a.a. ~ 151 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE3A
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 100646 DA
Gene ID 12406
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence
Comment

Background: This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Abaied, Trabelsi, Chaabouni et al.: "A novel UBE3A truncating mutation in large Tunisian Angelman syndrome pedigree." in: American journal of medical genetics. Part A, Vol. 152A, Issue 1, pp. 141-6, 2009 (PubMed).

Purbeck, Eletr, Kuhlman: "Kinetics of the transfer of ubiquitin from UbcH7 to E6AP." in: Biochemistry, Vol. 49, Issue 7, pp. 1361-3, 2010 (PubMed).

Sanduja, Kaza, Dixon: "The mRNA decay factor tristetraprolin (TTP) induces senescence in human papillomavirus-transformed cervical cancer cells by targeting E6-AP ubiquitin ligase." in: Aging, Vol. 1, Issue 9, pp. 803-17, 2010 (PubMed).

Wang, Mo, Ren et al.: "Activating transcription factor 3 activates p53 by preventing E6-associated protein from binding to E6." in: The Journal of biological chemistry, Vol. 285, Issue 17, pp. 13201-10, 2010 (PubMed).

Zaaroor-Regev, de Bie, Scheffner et al.: "Regulation of the polycomb protein Ring1B by self-ubiquitination or by E6-AP may have implications to the pathogenesis of Angelman syndrome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 15, pp. 6788-93, 2010 (PubMed).

Alternatives for antigen "Ubiquitin Protein Ligase E3A (ube3a)", type "Antibodies"
Hosts (46), (4)
Reactivities (48), (23), (16), (14), (12), (12), (3), (2), (1)
Applications (38), (14), (11), (10), (9), (8), (6), (5), (2)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (5), (5), (2), (2), (1), (1), (1), (1)
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