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Asparagine-Linked Glycosylation 6, alpha-1,3-Glucosyltransferase Homolog (S. Cerevisiae) (ALG6) (AA 25-115) antibody

Details for Product No. ABIN393620, Supplier: Log in to see
Antigen
  • CG5091
  • Dalg6
  • Dmel\\CG5091
  • alg6
  • zgc:100813
  • CDG1C
  • E230028F23Rik
Epitope
AA 25-115
5
3
2
2
Reactivity
Human
19
11
11
3
3
3
2
2
Host
Mouse
14
5
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
18
10
1
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Immunogen ALG6 (NP_037471, 25 a.a. ~ 115 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2G11
Isotype IgG2a kappa
Specificity ALG6 (NP_037471, 25 a.a. ~ 115 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ALG6 (ALG6 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 58181 DA
Gene ID 12406
NCBI Accession NP_037471
Research Area Protein Modifications, Cell Structure
Pathways
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the ALG6/ALG8 glucosyltransferase family. The encoded protein catalyzes the addition of the first glucose residue to the growing lipid-linked oligosaccharide precursor of N-linked glycosylation. Mutations in this gene are associated with congenital disorders of glycosylation type Ic.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Asparagine-Linked Glycosylation 6, alpha-1,3-Glucosyltransferase Homolog (S. Cerevisiae) (ALG6) (AA 25-115) antibody (ABIN393620) ALG6 monoclonal antibody (M09), clone 2G11 Western Blot analysis of ALG6 expression i...
Background publications Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1. ..." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Eklund, Sun, Yang et al.: "Congenital disorder of glycosylation Ic due to a de novo deletion and an hALG-6 mutation." in: Biochemical and biophysical research communications, Vol. 339, Issue 3, pp. 755-60, 2005 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Westphal, Xiao, Kwok et al.: "Identification of a frequent variant in ALG6, the cause of Congenital Disorder of Glycosylation-Ic." in: Human mutation, Vol. 22, Issue 5, pp. 420-1, 2003 (PubMed).

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