Hect Domain and RLD 4 (HERC4) (AA 341-441) antibody

Details for Product No. ABIN393628
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Antigen
Synonyms 1700056O17Rik, 4921531D01Rik, 9530080M15Rik, mKIAA1593
Epitope
AA 341-441
(5), (4), (2), (2), (2), (1)
Reactivity
Human
(33), (18), (7), (2), (2), (2)
Host
Mouse
(30), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(24), (10), (7), (3), (3), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393628
450.00 $
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Immunogen HERC4 (NP_071362, 341 a.a. ~ 441 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2G7
Isotype IgG2a kappa
Specificity HERC4 (NP_071362, 341 a.a. ~ 441 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name HERC4
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 118563 DA
Gene ID 12406
NCBI Accession NP_071362
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Hect Domain and RLD 4 (HERC4) (AA 341-441) antibody Detection limit for recombinant GST tagged HERC4 is approximately 0.3 ng/mL as a capture antibody.
Background publications Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Hochrainer, Mayer, Baranyi et al.: "The human HERC family of ubiquitin ligases: novel members, genomic organization, expression profiling, and evolutionary aspects." in: Genomics, Vol. 85, Issue 2, pp. 153-64, 2005 (PubMed).

Grupe, Li, Rowland et al.: "A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease." in: American journal of human genetics, Vol. 78, Issue 1, pp. 78-88, 2005 (PubMed).

Hochrainer, Kroismayr, Baranyi et al.: "Highly homologous HERC proteins localize to endosomes and exhibit specific interactions with hPLIC and Nm23B." in: Cellular and molecular life sciences : CMLS, Vol. 65, Issue 13, pp. 2105-17, 2008 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

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