Hect Domain and RLD 4 (HERC4) antibody

Antigen: Hect Domain and RLD 4 (HERC4)

Synonyms: KIAA1593, DKFZp564G092, mKIAA1593, 1700056O17Rik, 4921531D01Rik, 9530080M15Rik, MGC81587, MGC132293, HERC4, MGC142977

Clonality: Monoclonal (2G7)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393628

Additional Information

Alternative name: HERC4

Gene ID: 26091

UniProt: NP_071362

Immunogen: HERC4 (NP_071362, 341 a.a. ~ 441 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2G7

Description: Other names: DKFZP564G092, KIAA1593 hect domain and RLD 4

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: HERC4 (NP_071362, 341 a.a. ~ 441 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 118563 DA

Comments:

Background: HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged HERC4 is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Hochrainer, Mayer, Baranyi et al.: "The human HERC family of ubiquitin ligases: novel members, genomic organization, expression profiling, and evolutionary aspects." in: Genomics, Vol. 85, Issue 2, pp. 153-64, 2005 (PubMed).

Grupe, Li, Rowland et al.: "A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease." in: American journal of human genetics, Vol. 78, Issue 1, pp. 78-88, 2005 (PubMed).

Hochrainer, Kroismayr, Baranyi et al.: "Highly homologous HERC proteins localize to endosomes and exhibit specific interactions with hPLIC and Nm23B." in: Cellular and molecular life sciences : CMLS, Vol. 65, Issue 13, pp. 2105-17, 2008 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).