Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibody

Antigen: Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B)

Synonyms: SSK1, BUBR1, Bub1A, MAD3L, hBUBR1, BUB1beta, Bub1b, BUB1B, bub1b, Bub1a, C80208, D2Xrf87, AL022991, AU045529, MGC36092, BUB1, BUB1-like, BubR1, CG14030/Bub1, DmelCG14030, CG14030, 31/13, BcDNA:LD23835, CG 7838, DmBUB1, bubR1, dBUB1, l(2)k03113, l(2)k06109, DmelCG7838, CG7838

Clonality: Monoclonal (2G9)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393631

Additional Information

Alternative name: BUB1B

Gene ID: 701

Immunogen: BUB1B (AAH18739, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG Mix kappa

Clone: 2G9

Description: Other names: BUB1beta, BUBR1, Bub1A, MAD3L, SSK1, hBUBR1 BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BUB1B (AAH18739, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 119545 DA

Comments:

Background: This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

Synonyms: SSK1, BUBR1, Bub1A, MAD3L, hBUBR1, BUB1beta, Bub1b, BUB1B, bub1b, Bub1a, C80208, D2Xrf87, AL022991, AU045529, MGC36092, BUB1, BUB1-like, BubR1, CG14030/Bub1, DmelCG14030, CG14030, 31/13, BcDNA:LD23835, CG 7838, DmBUB1, bubR1, dBUB1, l(2)k03113, l(2)k06109, DmelCG7838, CG7838

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to BUB1B (AT1320a) on HeLa cell . [antibody concentration 10 ug/ml] Immunoperoxidase of monoclonal antibody to BUB1B ( Cat # AT1320a ) on formalin-fixed paraffin-embedded human spleen. [antibody concentration 1 ug/ml]

anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibody (Image 2)

Publications

Product: Lv, Bu, Jiao et al.: "LSD1 is required for chromosome segregation during mitosis." in: European journal of cell biology, Vol. 89, Issue 7, pp. 557-63, 2010 (PubMed).

Lira, Miranda, Guimarães et al.: "BUBR1 expression in benign oral lesions and squamous cell carcinomas: correlation with human papillomavirus." in: Oncology reports, Vol. 23, Issue 4, pp. 1027-36, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Gladhaug, Westgaard, Schjølberg et al.: "Spindle proteins in resected pancreatic head adenocarcinomas: BubR1 is an independent prognostic factor in pancreatobiliary-type tumours." in: Histopathology, Vol. 56, Issue 3, pp. 345-55, 2010 (PubMed).

Suijkerbuijk, van Osch, Bos et al.: "Molecular causes for BUBR1 dysfunction in the human cancer predisposition syndrome mosaic variegated aneuploidy." in: Cancer research, Vol. 70, Issue 12, pp. 4891-900, 2010 (PubMed).