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Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 2-66) antibody

Details for Product No. ABIN393637
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Antigen
Synonyms marcks, MGC89127, MARCKS, Macs, PKCSL, KINC, macs, wu:fb55c06, wu:fb92d04, wu:fc19b04, wu:fc44a10, zgc:110235, 80K-L, MACS, PRKCSL
Epitope
AA 2-66
(26), (26), (25), (24), (23), (13), (8), (7), (6), (5), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(194), (138), (128), (54), (40), (36), (25), (3), (3), (1), (1), (1)
Host
Mouse
(195), (16), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
Western Blotting (WB)
(156), (59), (52), (40), (27), (26), (20), (4), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN393637
450.00 $
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Immunogen MARCKS (NP_002347, 2 a.a. ~ 66 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2H4
Isotype IgG1 kappa
Specificity MARCKS (NP_002347, 2 a.a. ~ 66 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MARCKS
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 31555 DA
Gene ID 12406
UniProt NP_002347
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 2-66) antibody MARCKS monoclonal antibody (M04), clone 2H4. Western Blot analysis of MARCKS expression in Raw 264.7. Detection limit for recombinant GST tagged MARCKS is approximately 0.1 ng/mL as a capture antibody.
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 2-66) antibody (2) anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 2-66) antibody (Image 2)
Background publications Eckert, Neuder, Park et al.: "Myristoylated alanine-rich C-kinase substrate (MARCKS) protein regulation of human neutrophil migration." in: American journal of respiratory cell and molecular biology, Vol. 42, Issue 5, pp. 586-94, 2010 (PubMed).

Micallef, Taccone, Mukherjee et al.: "Epidermal growth factor receptor variant III-induced glioma invasion is mediated through myristoylated alanine-rich protein kinase C substrate overexpression." in: Cancer research, Vol. 69, Issue 19, pp. 7548-56, 2009 (PubMed).

Finlayson, Freeman: "A cell motility screen reveals role for MARCKS-related protein in adherens junction formation and tumorigenesis." in: PLoS ONE, Vol. 4, Issue 11, pp. e7833, 2009 (PubMed).

Techasen, Loilome, Namwat et al.: "Myristoylated alanine-rich C kinase substrate phosphorylation promotes cholangiocarcinoma cell migration and metastasis via the protein kinase C-dependent pathway." in: Cancer science, Vol. 101, Issue 3, pp. 658-65, 2010 (PubMed).

Lin, Fang, Park et al.: "MARCKS and related chaperones bind to unconventional myosin V isoforms in airway epithelial cells." in: American journal of respiratory cell and molecular biology, Vol. 43, Issue 2, pp. 131-6, 2010 (PubMed).

Validation Images
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