NEDD4 Binding Protein 2 (N4BP2) antibody

Antigen: NEDD4 Binding Protein 2 (N4BP2)

Synonyms: B3BP, FLJ10680, KIAA1413, B3bp, Gm868, Gm1791, E430014I16

Clonality: Monoclonal (2H5)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393639

Additional Information

Alternative name: N4BP2

Gene ID: 55728

UniProt: NP_060647

Immunogen: N4BP2 (NP_060647, 1671 a.a. ~ 1771 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 2H5

Description: Other names: B3BP, FLJ10680, KIAA1413 Nedd4 binding protein 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: N4BP2 (NP_060647, 1671 a.a. ~ 1771 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 198801 DA

Comments:

Background: This gene encodes a protein containing a polynucleotide kinase domain (PNK) near the N-terminal region, and a Small MutS Related (Smr) domain near the C-terminal region. The encoded protein can bind to both B-cell leukemia/lymphoma 3 (BCL-3) and neural precursor cell expressed, developmentally downregulated 4, (Nedd4) proteins. This protein binds and hydrolyzes ATP, may function as a 5'-polynucleotide kinase, and has the capacity to be a ubiquitylation substrate. This protein may play a role in transcription-coupled DNA repair or genetic recombination.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged N4BP2 is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Zheng, Qin, Yu et al.: "Haplotype of gene Nedd4 binding protein 2 associated with sporadic nasopharyngeal carcinoma in the Southern Chinese population." in: Journal of translational medicine, Vol. 5, pp. 36, 2007 (PubMed).

Lee, Villa, Trojer et al.: "Demethylation of H3K27 regulates polycomb recruitment and H2A ubiquitination." in: Science (New York, N.Y.), Vol. 318, Issue 5849, pp. 447-50, 2007 (PubMed).

Oshikawa, Sugai, Usami et al.: "Fine expression profiling of full-length transcripts using a size-unbiased cDNA library prepared with the vector-capping method." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 15, Issue 3, pp. 123-36, 2008 (PubMed).

Diercks, AB, Daniels et al.: "Solution structure and characterization of the DNA-binding activity of the B3BP-Smr domain." in: Journal of molecular biology, Vol. 383, Issue 5, pp. 1156-70, 2008 (PubMed).