Apoptosis Antagonizing Transcription Factor (AATF) antibody

Details for Product No. ABIN393641
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Antigen
Synonyms DED, CHE1, CHE-1, 4933415H02Rik, 5830465M17Rik, Che-1, Trb, Ded, AATF, ded, che1, che-1, cb109, sb:cb109, wu:fc13d05, zgc:162071
Reactivity
Human
(59), (34), (26), (14), (12), (12), (3), (2), (1)
Host
Mouse
(50), (11)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(47), (30), (15), (13), (10), (6), (2), (2), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN393641
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen AATF (AAH00591, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2H6
Isotype IgG1 kappa
Specificity AATF (AAH00591, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AATF
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 63133 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Apoptosis Antagonizing Transcription Factor (AATF) antibody Immunofluorescence of monoclonal antibody to AATF on HeLa cell (antibody concentration 10 µg/mL). AATF monoclonal antibody (M09), clone 2H6 Western Blot analysis of AATF expression in HeLa.
anti-Apoptosis Antagonizing Transcription Factor (AATF) antibody (2) anti-Apoptosis Antagonizing Transcription Factor (AATF) antibody (Image 2)
Background publications Kaul: "Cellular AATF gene: armour against HIV-1." in: Indian journal of biochemistry & biophysics, Vol. 44, Issue 5, pp. 276-8, 2008 (PubMed).

Wheeler, Metter, Tanaka et al.: "Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging." in: PLoS genetics, Vol. 5, Issue 10, pp. e1000685, 2009 (PubMed).

Haanpää, Reiman, Nikkilä et al.: "Mutation analysis of the AATF gene in breast cancer families." in: BMC cancer, Vol. 9, pp. 457, 2010 (PubMed).

Naukkarinen, Surakka, Pietiläinen et al.: "Use of genome-wide expression data to mine the "Gray Zone" of GWA studies leads to novel candidate obesity genes." in: PLoS genetics, Vol. 6, Issue 6, pp. e1000976, 2010 (PubMed).

Bruno, Desantis, Bossi et al.: "Che-1 promotes tumor cell survival by sustaining mutant p53 transcription and inhibiting DNA damage response activation." in: Cancer cell, Vol. 18, Issue 2, pp. 122-34, 2010 (PubMed).

Hosts (50), (11)
Reactivities (59), (34), (26), (14), (12), (12), (3), (2), (1)
Applications (47), (30), (15), (13), (10), (6), (2), (2), (1), (1), (1)
Conjugates (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (11), (7), (4), (1), (1), (1), (1), (1)
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