Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (AA 694-794) antibody

Details for Product No. ABIN393646
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Antigen
Synonyms UHRF1, ICBP90, Np95, RNF106, hNP95, hUHRF1, NP95, fb97f09, wu:fb97f09, zgc:63539, AL022808, C6orf107, dJ349A12.1
Epitope
AA 694-794
(7), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(52), (15), (13), (11), (11), (11), (11), (3), (1)
Host
Mouse
(38), (16), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(46), (21), (18), (9), (8), (7), (7), (6), (6), (4), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393646
450.00 $
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Immunogen UHRF1 (NP_037414, 694 a.a. ~ 794 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3A11
Isotype IgG1 kappa
Specificity UHRF1 (NP_037414, 694 a.a. ~ 794 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UHRF1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 89814 DA
Gene ID 12406
UniProt NP_037414
Research Area Ubiquitin-related antibodies, Signaling, Protein Modifications, Cell Cycle, Chromatin, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (AA 694-794) antibody Immunofluorescence of monoclonal antibody to UHRF1 on HeLa cell (antibody concentration 10 µg/mL). UHRF1 monoclonal antibody (M01), clone 3A11 Western Blot analysis of UHRF1 expression in HeLa NE.
anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (AA 694-794) antibody (2) anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (AA 694-794) antibody (Image 2)
Background publications Meilinger, Fellinger, Bultmann et al.: "Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells." in: EMBO reports, Vol. 10, Issue 11, pp. 1259-64, 2009 (PubMed).

Achour, Fuhrmann, Alhosin et al.: "UHRF1 recruits the histone acetyltransferase Tip60 and controls its expression and activity." in: Biochemical and biophysical research communications, Vol. 390, Issue 3, pp. 523-8, 2009 (PubMed).

Oguri, Kato, Yokoi et al.: "Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals." in: American journal of hypertension, Vol. 23, Issue 1, pp. 70-7, 2009 (PubMed).

Yoshida, Kato, Yokoi et al.: "Association of genetic variants with hemorrhagic stroke in Japanese individuals." in: International journal of molecular medicine, Vol. 25, Issue 4, pp. 649-56, 2010 (PubMed).

Hervouet, Lalier, Debien et al.: "Disruption of Dnmt1/PCNA/UHRF1 interactions promotes tumorigenesis from human and mice glial cells." in: PLoS ONE, Vol. 5, Issue 6, pp. e11333, 2010 (PubMed).

Validation Images
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