Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) antibody

Antigen: Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1)

Synonyms: Np95, hNP95, ICBP90, RNF106, FLJ21925, MGC138707, AL022808, Ac2-121, NP95, fb97f09, zgc:63539, wu:fb97f09, UHRF1, MGC166355

Clonality: Monoclonal (3A11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393646

Additional Information

Alternative name: UHRF1

Gene ID: 29128

UniProt: NP_037414

Immunogen: UHRF1 (NP_037414, 694 a.a. ~ 794 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3A11

Description: Other names: FLJ21925, ICBP90, Np95, RNF106, huNp95 ubiquitin-like, containing PHD and RING finger domains, 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: UHRF1 (NP_037414, 694 a.a. ~ 794 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 89814 DA

Comments:

Background: This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Signaling, Protein Modifications, Cell Cycle, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to UHRF1 (AT4461a) on HeLa cell . [antibody concentration 10 ug/ml] UHRF1 monoclonal antibody (M01), clone 3A11 Western Blot analysis of UHRF1 expression in HeLa NE ( Cat # L013V3 ).

anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) antibody (Image 2)

Publications

Product: Meilinger, Fellinger, Bultmann et al.: "Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells." in: EMBO reports, Vol. 10, Issue 11, pp. 1259-64, 2009 (PubMed).

Achour, Fuhrmann, Alhosin et al.: "UHRF1 recruits the histone acetyltransferase Tip60 and controls its expression and activity." in: Biochemical and biophysical research communications, Vol. 390, Issue 3, pp. 523-8, 2009 (PubMed).

Oguri, Kato, Yokoi et al.: "Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals." in: American journal of hypertension, Vol. 23, Issue 1, pp. 70-7, 2009 (PubMed).

Yoshida, Kato, Yokoi et al.: "Association of genetic variants with hemorrhagic stroke in Japanese individuals." in: International journal of molecular medicine, Vol. 25, Issue 4, pp. 649-56, 2010 (PubMed).

Hervouet, Lalier, Debien et al.: "Disruption of Dnmt1/PCNA/UHRF1 interactions promotes tumorigenesis from human and mice glial cells." in: PLoS ONE, Vol. 5, Issue 6, pp. e11333, 2010 (PubMed).