Cyclin D2 (CCND2) antibody

Antigen: Cyclin D2 (CCND2)

Synonyms: KIAK0002, MGC102758, cD2, Vin1, Vin-1, C86853, AI256817, BF642806, 2600016F06Rik, ccnd2-A, CCND2, si:ch211-240j22.2, MGC142404, MGC132398, ccnd2, kiak0002, LOC100224096

Clonality: Monoclonal (3B10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393662

Additional Information

Alternative name: CCND2

Gene ID: 894

Immunogen: CCND2 (AAH10958, 190 a.a. ~ 289 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3B10

Description: Other names: KIAK0002, MGC102758 cyclin D2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CCND2 (AAH10958, 190 a.a. ~ 289 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 33067 DA

Comments:

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of CCND2 expression in transfected 293T cell line by CCND2 monoclonal antibody (M01), clone 3B10. Lane 1: CCND2 transfected lysate(33.1 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Becker, Ghule, Lian et al.: "Cyclin D2 and the CDK substrate p220(NPAT) are required for self-renewal of human embryonic stem cells." in: Journal of cellular physiology, Vol. 222, Issue 2, pp. 456-64, 2009 (PubMed).

Kosmaczewska, Ciszak, Szteblich et al.: "Is cyclin D2 a marker of B-cLL cell activation?" in: Oncology research, Vol. 18, Issue 2-3, pp. 127-31, 2010 (PubMed).

Park, Chun, Bae et al.: "CCND2 polymorphisms associated with clearance of HBV infection." in: Journal of human genetics, Vol. 55, Issue 7, pp. 416-20, 2010 (PubMed).

Johnatty, Beesley, Chen et al.: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010 (PubMed).

Shimada, Miyagawa, Kawashima et al.: "An approach based on a genome-wide association study reveals candidate loci for narcolepsy." in: Human genetics, Vol. 128, Issue 4, pp. 433-41, 2010 (PubMed).