Vaccinia Related Kinase 2 (VRK2) antibody

Antigen: Vaccinia Related Kinase 2 (VRK2)

Clonality: Monoclonal (3B10)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393663

Additional Information

Alternative name: VRK2

Gene ID: 7444

Immunogen: VRK2 (AAH27854, 260 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3B10

Description: Other names: vaccinia related kinase 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: VRK2 (AAH27854, 260 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 58141 DA

Comments:

Background: This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, leukocytes, fetal liver, and carcinomas. Its protein localizes to the endoplasmic reticulum and has been shown to phosphorylate casein and undergo autophosphorylation. Multiple alternatively spliced transcript variants have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to VRK2 (AT4522a) on HeLa cell . [antibody concentration 10 ug/ml] Western blot analysis of VRK2 over-expressed 293 cell line, cotransfected with VRK2 Validated Chimera RNAi ( Cat # H00007444-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with VRK2 monoclonal antibody (M01) clone 3B10 (Cat # AT4522a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-Vaccinia Related Kinase 2 (VRK2) antibody (Image 2)

Publications

Product: Blanco, Santos, Lazo: "Vaccinia-related kinase 2 modulates the stress response to hypoxia mediated by TAK1." in: Molecular and cellular biology, Vol. 27, Issue 20, pp. 7273-83, 2007 (PubMed).

Blanco, Sanz-García, Santos et al.: "Modulation of interleukin-1 transcriptional response by the interaction between VRK2 and the JIP1 scaffold protein." in: PLoS ONE, Vol. 3, Issue 2, pp. e1660, 2008 (PubMed).

Valbuena, Blanco, Vega et al.: "The C/H3 domain of p300 is required to protect VRK1 and VRK2 from their downregulation induced by p53." in: PLoS ONE, Vol. 3, Issue 7, pp. e2649, 2008 (PubMed).

Sanz-García, López-Sánchez, Lazo: "Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 11, pp. 2199-214, 2008 (PubMed).

Stefansson, Ophoff, Steinberg et al.: "Common variants conferring risk of schizophrenia." in: Nature, Vol. 460, Issue 7256, pp. 744-7, 2009 (PubMed).