Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) (AA 21-130) antibody

Details for Product No. ABIN393664
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Antigen
Synonyms CDC25C, cdc25, xcdc25c, CDC25, PPP1R60, Cdc25, cdc25-3, xcdc25-3
Epitope
AA 21-130
(37), (18), (11), (9), (6), (6), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(202), (32), (31), (12)
Host
Mouse
(135), (66), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Application
Western Blotting (WB)
(142), (74), (71), (38), (31), (30), (27), (15), (6), (4), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN393664
450.00 $
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Immunogen CDC25C (AAH19089, 21 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3B11
Isotype IgG1 kappa
Specificity CDC25C (AAH19089, 21 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CDC25C
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 53365 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) (AA 21-130) antibody Western Blot analysis of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody (M01), clone 3B11. Lane 1: CDC25C transfected lysate(53.312 KDa). Lane 2: Non-transfected lysate. Western blot analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi used as specificity and loading control.
anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) (AA 21-130) antibody (2) anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) (AA 21-130) antibody (Image 2)
Background publications Bhattacharjee, Sheng, Ajees et al.: "Adventitious arsenate reductase activity of the catalytic domain of the human Cdc25B and Cdc25C phosphatases." in: Biochemistry, Vol. 49, Issue 4, pp. 802-9, 2010 (PubMed).

Timofeev, Cizmecioglu, Settele et al.: "Cdc25 phosphatases are required for timely assembly of CDK1-cyclin B at the G2/M transition." in: The Journal of biological chemistry, Vol. 285, Issue 22, pp. 16978-90, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Wang, Trope, Flørenes et al.: "Overexpression of CDC25B, CDC25C and phospho-CDC25C (Ser216) in vulvar squamous cell carcinomas are associated with malignant features and aggressive cancer phenotypes." in: BMC cancer, Vol. 10, pp. 233, 2010 (PubMed).

Olson, Wang, Pankratz et al.: "Centrosome-related genes, genetic variation, and risk of breast cancer." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 221-8, 2010 (PubMed).

Validation Images
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