Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) antibody

Antigen: Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C)

Synonyms: Cdc25, CDC25C, CDC25, cdc25, xcdc25c

Clonality: Monoclonal (3B11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393664

Additional Information

Alternative name: CDC25C

Gene ID: 995

Immunogen: CDC25C (AAH19089, 21 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3B11

Description: Other names: CDC25 cell division cycle 25C

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CDC25C (AAH19089, 21 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 53365 DA

Comments:

Background: This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody (M01), clone 3B11. Lane 1: CDC25C transfected lysate(53.312 KDa). Lane 2: Non-transfected lysate. Western blot analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi ( Cat # H00000995-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody (M01), clone 3B11 (Cat # AT1458a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) antibody (Image 2)

Publications

Product: Bhattacharjee, Sheng, Ajees et al.: "Adventitious arsenate reductase activity of the catalytic domain of the human Cdc25B and Cdc25C phosphatases." in: Biochemistry, Vol. 49, Issue 4, pp. 802-9, 2010 (PubMed).

Timofeev, Cizmecioglu, Settele et al.: "Cdc25 phosphatases are required for timely assembly of CDK1-cyclin B at the G2/M transition." in: The Journal of biological chemistry, Vol. 285, Issue 22, pp. 16978-90, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Wang, Trope, Flørenes et al.: "Overexpression of CDC25B, CDC25C and phospho-CDC25C (Ser216) in vulvar squamous cell carcinomas are associated with malignant features and aggressive cancer phenotypes." in: BMC cancer, Vol. 10, pp. 233, 2010 (PubMed).

Olson, Wang, Pankratz et al.: "Centrosome-related genes, genetic variation, and risk of breast cancer." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 221-8, 2010 (PubMed).