Linker For Activation of T Cells (LAT) antibody

Antigen: Linker For Activation of T Cells (LAT)

Synonyms: LAT1, pp36, MGC108666, LAT, MGC157208, lat

Clonality: Monoclonal (3B11-1D5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393665

Additional Information

Alternative name: LAT

Gene ID: 27040

Immunogen: LAT (AAH11563, 1 a.a. ~ 234 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3B11-1D5

Description: Other names: linker for activation of T cells

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: LAT (AAH11563, 1 a.a. ~ 234 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: NONE?! DA

Comments:

Background: The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

LAT monoclonal antibody (M01), clone 3B11-1D5 Western Blot analysis of LAT expression in HL-60 ( Cat # L014V1 ). LAT monoclonal antibody (M01), clone 3B11-1D5. Western Blot analysis of LAT expression in HeLa ( Cat # L013V1 ).

anti-Linker For Activation of T Cells (LAT) antibody (Image 2)

Publications

Product: Saito, Kawamoto, Kamatani: "Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects." in: Journal of human genetics, Vol. 54, Issue 6, pp. 317-23, 2009 (PubMed).

Shen, Zhu, Lau et al.: "The essential role of LAT in thymocyte development during transition from the double-positive to single-positive stage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 9, pp. 5596-604, 2009 (PubMed).

Cruz-Orcutt, Houtman: "PI3 kinase function is vital for the function but not formation of LAT-mediated signaling complexes." in: Molecular immunology, Vol. 46, Issue 11-12, pp. 2274-83, 2009 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).