MAP/microtubule Affinity-Regulating Kinase 2 (MARK2) antibody

Antigen: MAP/microtubule Affinity-Regulating Kinase 2 (MARK2)

Synonyms: EMK1, PAR-1, Par1b, MGC99619

Clonality: Monoclonal (3B12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393666

Additional Information

Alternative name: MARK2

Gene ID: 2011

Immunogen: MARK2 (AAH08771, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3B12

Description: Other names: EMK1, MGC99619, PAR1MAP/microtubule affinity-regulating kinase 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: MARK2 (AAH08771, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 87911 DA

Comments:

Background: This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged MARK2 is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Motovska, Widimsky, Kvasnicka et al.: "High loading dose of clopidogrel is unable to satisfactorily inhibit platelet reactivity in patients with glycoprotein IIIA gene polymorphism: a genetic substudy of PRAGUE-8 trial." in: Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis, Vol. 20, Issue 4, pp. 257-62, 2009 (PubMed).

Lin, Hou, Piwnica-Worms et al.: "The polarity protein Par1b/EMK/MARK2 regulates T cell receptor-induced microtubule-organizing center polarization." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 2, pp. 1215-21, 2009 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Yamashita, Suzuki, Satoh et al.: "The 8th and 9th tandem spectrin-like repeats of utrophin cooperatively form a functional unit to interact with polarity-regulating kinase PAR-1b." in: Biochemical and biophysical research communications, Vol. 391, Issue 1, pp. 812-7, 2010 (PubMed).

Yoshimura, Terabayashi, Miki: "Par1b/MARK2 phosphorylates kinesin-like motor protein GAKIN/KIF13B to regulate axon formation." in: Molecular and cellular biology, Vol. 30, Issue 9, pp. 2206-19, 2010 (PubMed).