Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (AA 1-155) antibody

Details for Product No. ABIN393672
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Antigen
Synonyms UBE2L3, E2-F1, L-UBC, UBCH7, UbcM4, C79827, Ubce7, ube2l3l, wu:fb38h03
Epitope
AA 1-155
(23), (10), (9), (6), (3), (2), (2), (2), (1), (1)
Reactivity
Human
(68), (29), (27), (17), (4), (3), (3), (3), (3), (1)
Host
Mouse
(54), (8), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(58), (31), (15), (13), (10), (9), (7), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393672
450.00 $
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Immunogen UBE2L3 (AAH53368, 1 a.a. ~ 155 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3B7
Isotype IgG2a kappa
Specificity UBE2L3 (AAH53368, 1 a.a. ~ 155 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2L3
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 17862 DA
Gene ID 12406
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (AA 1-155) antibody Immunofluorescence of monoclonal antibody to UBE2L3 on HeLa cell (antibody concentration 10 µg/mL). UBE2L3 monoclonal antibody (M01), clone 3B7 Western Blot analysis of UBE2L3 expression in K-562.
anti-Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (AA 1-155) antibody (2) anti-Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (AA 1-155) antibody (Image 2)
Background publications Huang, de Jong, Wienk et al.: "E2-c-Cbl recognition is necessary but not sufficient for ubiquitination activity." in: Journal of molecular biology, Vol. 385, Issue 2, pp. 507-19, 2009 (PubMed).

Han, Zheng, Cui et al.: "Genome-wide association study in a Chinese Han population identifies nine new susceptibility loci for systemic lupus erythematosus...." in: Nature genetics, Vol. 41, Issue 11, pp. 1234-7, 2009 (PubMed).

Purbeck, Eletr, Kuhlman: "Kinetics of the transfer of ubiquitin from UbcH7 to E6AP." in: Biochemistry, Vol. 49, Issue 7, pp. 1361-3, 2010 (PubMed).

Dubois, Trynka, Franke et al.: "Multiple common variants for celiac disease influencing immune gene expression...." in: Nature genetics, Vol. 42, Issue 4, pp. 295-302, 2010 (PubMed).

Fransen, Visschedijk, van Sommeren et al.: "Analysis of SNPs with an effect on gene expression identifies UBE2L3 and BCL3 as potential new risk genes for Crohn's disease." in: Human molecular genetics, Vol. 19, Issue 17, pp. 3482-8, 2010 (PubMed).

Validation Images
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