Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3)

Synonyms: E2-F1, L-UBC, UBCH7, UbcM4, Ubce7, C79827, MGC118100, zgc:86727, wu:fc22h11, MGC114833, MGC132024, UBE2L3, MGC127956, DKFZp469G1720

Clonality: Monoclonal (3B7)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393672

Additional Information

Alternative name: UBE2L3

Gene ID: 7332

Immunogen: UBE2L3 (AAH53368, 1 a.a. ~ 155 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3B7

Description: Other names: E2F1, LUBC, UBCH7, UbcM4 ubiquitin-conjugating enzyme E2L 3

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: UBE2L3 (AAH53368, 1 a.a. ~ 155 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 17862 DA

Comments:

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to UBE2L3 (AT4439a) on HeLa cell. [antibody concentration 10ug/ml] UBE2L3 monoclonal antibody (M01), clone 3B7 Western Blot analysis of UBE2L3 expression in K-562 ( Cat # L009V1 ).

anti-Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) antibody (Image 2)

Publications

Product: Huang, de Jong, Wienk et al.: "E2-c-Cbl recognition is necessary but not sufficient for ubiquitination activity." in: Journal of molecular biology, Vol. 385, Issue 2, pp. 507-19, 2009 (PubMed).

Han, Zheng, Cui et al.: "Genome-wide association study in a Chinese Han population identifies nine new susceptibility loci for systemic lupus erythematosus." in: Nature genetics, Vol. 41, Issue 11, pp. 1234-7, 2009 (PubMed).

Purbeck, Eletr, Kuhlman: "Kinetics of the transfer of ubiquitin from UbcH7 to E6AP." in: Biochemistry, Vol. 49, Issue 7, pp. 1361-3, 2010 (PubMed).

Dubois, Trynka, Franke et al.: "Multiple common variants for celiac disease influencing immune gene expression." in: Nature genetics, Vol. 42, Issue 4, pp. 295-302, 2010 (PubMed).

Fransen, Visschedijk, van Sommeren et al.: "Analysis of SNPs with an effect on gene expression identifies UBE2L3 and BCL3 as potential new risk genes for Crohn's disease." in: Human molecular genetics, Vol. 19, Issue 17, pp. 3482-8, 2010 (PubMed).