Cyclin K (CCNK) antibody

Antigen: Cyclin K (CCNK)

Synonyms: CPR4, MGC9113, CycK, AW123198, AW413594, DmelCG15218, CG15218, CCNK, fe49g08, MGC158372, wu:fe49g08, zgc:158372, si:dkey-60a16.2, MGC115029, cpr4, MGC145994

Clonality: Monoclonal (3B7-1B9)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393673

Additional Information

Alternative name: CCNK

Gene ID: 8812

Immunogen: CCNK (AAH15935, 1 a.a. ~ 355 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3B7-1B9

Description: Other names: CPR4, MGC9113 cyclin K

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: CCNK (AAH15935, 1 a.a. ~ 355 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 41293 DA

Comments:

Background: The protein encoded by this gene is a member of the transcription cyclin family. These cyclins may regulate transcription through their association with and activation of cyclin-dependent kinases (CDK) that phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II. This gene product may play a dual role in regulating CDK and RNA polymerase II activities.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of CCNK expression in transfected 293T cell line by CCNK monoclonal antibody (M01), clone 3B7-1B9. Lane 1: CCNK transfected lysate(41 KDa). Lane 2: Non-transfected lysate. Western blot analysis of CCNK over-expressed 293 cell line, cotransfected with CCNK Validated Chimera RNAi ( Cat # H00008812-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CCNK monoclonal antibody (M01), clone 3B7-1B9 (Cat # AT1423a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-Cyclin K (CCNK) antibody (Image 2)

Publications

Product: Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Lim, Hao, Shaw et al.: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Baek, Brown, Birrane et al.: "Crystal structure of human cyclin K, a positive regulator of cyclin-dependent kinase 9." in: Journal of molecular biology, Vol. 366, Issue 2, pp. 563-73, 2007 (PubMed).

Marsaud, Tchakarska, Andrieux et al.: "Cyclin K and cyclin D1b are oncogenic in myeloma cells." in: Molecular cancer, Vol. 9, pp. 103, 2010 (PubMed).