Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 1-101) antibody

Details for Product No. ABIN393677
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Antigen
Synonyms p40phox, AI451400, NCF, P40PHOX, SH3PXD4
Epitope
AA 1-101
(18), (10), (8), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(80), (44), (33), (30), (24), (14), (12), (2)
Host
Mouse
(69), (7), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
ELISA
(48), (26), (23), (20), (17), (5), (3), (2), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393677
450.00 $
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Immunogen NCF4 (AAH02798, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3C10
Isotype IgG2a kappa
Specificity NCF4 (AAH02798, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name NCF4
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 39032 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml
Comment

Background: The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 1-101) antibody Detection limit for recombinant GST tagged NCF4 is approximately 0.3 ng/mL as a capture antibody.
Background publications Segat, Brandão, Guimarães et al.: "Polymorphisms in innate immunity genes and patients response to dendritic cell-based HIV immuno-treatment." in: Vaccine, Vol. 28, Issue 10, pp. 2201-6, 2010 (PubMed).

Roos, Kuhns, Maddalena et al.: "Hematologically important mutations: the autosomal recessive forms of chronic granulomatous disease (second update)." in: Blood cells, molecules & diseases, Vol. 44, Issue 4, pp. 291-9, 2010 (PubMed).

Rajaraman, Brenner, Neta et al.: "Risk of meningioma and common variation in genes related to innate immunity." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 19, Issue 5, pp. 1356-61, 2010 (PubMed).

Han, Lan, Park et al.: "Polymorphisms in innate immunity genes and risk of childhood leukemia." in: Human immunology, Vol. 71, Issue 7, pp. 727-30, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

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