RAD23 Homolog A (S. Cerevisiae) (RAD23A) antibody

Antigen: RAD23 Homolog A (S. Cerevisiae) (RAD23A)

Synonyms: HHR23A, MGC111083, HR23A, mHR23A, AL024030, 2310040P19Rik, zgc:92001, wu:fe11h06, RAD23A, MGC140027

Clonality: Monoclonal (3C12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393680

Additional Information

Alternative name: RAD23A

Gene ID: 5886

Immunogen: RAD23A (AAH14026, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3C12

Description: Other names: HHR23A, MGC111083 RAD23 homolog A (S. cerevisiae)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: RAD23A (AAH14026, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 39609 DA

Comments:

Background: The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of RAD23A over-expressed 293 cell line, cotransfected with RAD23A Validated Chimera RNAi ( Cat # H00005886-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD23A monoclonal antibody (M01), clone 3C12 (Cat # AT3547a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of RAD23A expression in transfected 293T cell line by RAD23A monoclonal antibody (M01), clone 3C12. Lane 1: RAD23A transfected lysate(39.6 KDa). Lane 2: Non-transfected lysate.

anti-RAD23 Homolog A (S. Cerevisiae) (RAD23A) antibody (Image 2)

Publications

Product: Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Monsees, Kraft, Chanock et al.: "Comprehensive screen of genetic variation in DNA repair pathway genes and postmenopausal breast cancer risk." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 207-14, 2010 (PubMed).

Briggs, Goldstein, McCauley et al.: "Variation within DNA repair pathway genes and risk of multiple sclerosis." in: American journal of epidemiology, Vol. 172, Issue 2, pp. 217-24, 2010 (PubMed).

Li, Elder, Dubrovsky et al.: "HIV-1 replication through hHR23A-mediated interaction of Vpr with 26S proteasome." in: PLoS ONE, Vol. 5, Issue 6, pp. e11371, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).