F-Box and Leucine-Rich Repeat Protein 3 (FBXL3) antibody

Antigen: F-Box and Leucine-Rich Repeat Protein 3 (FBXL3)

Synonyms: FBL3, FBL3A, FBXL3A, FBK, Ovtm, Fbl3a, Fbxl3a, AU041772, AW212966, fbxl3, MGC80572, FBXL3, MGC151742, F7A7.240, F7A7_240

Clonality: Monoclonal (3C4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393683

Additional Information

Alternative name: FBXL3

Gene ID: 26224

UniProt: NP_036290

Immunogen: FBXL3 (NP_036290, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3C4

Description: Other names: FBL3, FBL3A, FBXL3A F-box and leucine-rich repeat protein 3

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: FBXL3 (NP_036290, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 48707 DA

Comments:

Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats and is localized in the nucleus.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged FBXL3 is approximately 1ng/ml as a capture antibody.

Publications

Product: Ilyin, Rialland, Pigeon et al.: "cDNA cloning and expression analysis of new members of the mammalian F-box protein family." in: Genomics, Vol. 67, Issue 1, pp. 40-7, 2000 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Busino, Bassermann, Maiolica et al.: "SCFFbxl3 controls the oscillation of the circadian clock by directing the degradation of cryptochrome proteins." in: Science (New York, N.Y.), Vol. 316, Issue 5826, pp. 900-4, 2007 (PubMed).