Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-184) antibody

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Antigen
Synonyms DDBDRAFT_0204633, DDBDRAFT_0238042, DDB_0204633, DDB_0238042, UBC-RS2, UBC12, hUbc12, 2510040H03Rik, Ubc-rs2
Epitope
AA 1-184
(5), (2), (2), (2), (2), (1), (1)
Reactivity
Human
(32), (18), (15), (4), (2), (1), (1), (1), (1)
Host
Mouse
(26), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(22), (12), (10), (9), (8), (5), (3), (2), (1), (1)
Pubmed 5 references available
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Quantity 0.1 mg
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Immunogen UBE2M (AAH58924, 1 a.a. ~ 184 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3C4
Isotype IgG1 kappa
Specificity UBE2M (AAH58924, 1 a.a. ~ 184 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2M (ube2m Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 20900 DA
Gene ID 12406
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-184) antibody (ABIN393685) Immunofluorescence of monoclonal antibody to UBE2M on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-184) antibody (ABIN393685) anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-184) antibody (Image 2)
Background publications Huang, Ayrault, Hunt et al.: "E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification." in: Molecular cell, Vol. 33, Issue 4, pp. 483-95, 2009 (PubMed).

Kim, Bommeljé, Lee et al.: "SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33211-20, 2008 (PubMed).

Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: , Vol. 3, pp. 89, 2007 (PubMed).

Li, Santockyte, Shen et al.: "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers." in: Archives of biochemistry and biophysics, Vol. 453, Issue 1, pp. 70-4, 2006 (PubMed).

Catalog No. ABIN393685
$450.00
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