Ubiquitin-Conjugating Enzyme E2M (ube2m) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2M (ube2m)

Synonyms: UBC12, hUbc12, UBC-RS2, Ubc-rs2, MGC25453, MGC37197, 2510040H03Rik, ube2m, MGC75750, MGC80512, UBE2M, MGC151985

Clonality: Monoclonal (3C4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393685

Additional Information

Alternative name: UBE2M

Gene ID: 9040

Immunogen: UBE2M (AAH58924, 1 a.a. ~ 184 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3C4

Description: Other names: UBCRS2, UBC12, hUbc12ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: UBE2M (AAH58924, 1 a.a. ~ 184 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 20900 DA

Comments:

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to UBE2M (AT4440a) on HeLa cell . [antibody concentration 10 ug/ml] UBE2M monoclonal antibody (M01), clone 3C4 Western Blot analysis of UBE2M expression in Jurkat ( Cat # L017V1 ).

anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) antibody (Image 2)

Publications

Product: Li, Santockyte, Shen et al.: "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers." in: Archives of biochemistry and biophysics, Vol. 453, Issue 1, pp. 70-4, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Kim, Bommeljé, Lee et al.: "SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33211-20, 2008 (PubMed).

Huang, Ayrault, Hunt et al.: "E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification." in: Molecular cell, Vol. 33, Issue 4, pp. 483-95, 2009 (PubMed).