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UBE2H antibody (Ubiquitin-Conjugating Enzyme E2H) (AA 1-184)

Details for Product anti-UBE2H Antibody No. ABIN393686, Supplier: Log in to see
Antigen
  • 1500009C23Rik
  • AI181839
  • AI326965
  • AI462483
  • AW227540
  • E2-20K
  • e2-20k
  • fc07a05
  • fi24c01
  • Gid3
  • GID3
  • N28148
  • si:bz3c13.2
  • Ubc8
  • UBC8
  • ubc8
  • UBCH
  • ubch
  • ubch2
  • UBCH2
  • wu:fc07a05
  • wu:fi24c01
Epitope
AA 1-184
16
15
14
13
10
3
2
2
1
1
Reactivity
Human
52
23
23
Host
Mouse
62
4
Clonality (Clone)
Monoclonal ()
Conjugate
This UBE2H antibody is un-conjugated
5
5
5
4
4
4
2
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
51
36
12
4
3
3
1
1
Supplier
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Immunogen UBE2H (AAH06277, 1 a.a. ~ 184 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3C4-1A2
Isotype IgG2a kappa
Specificity UBE2H (AAH06277, 1 a.a. ~ 184 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2H (UBE2H Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 20655 DA
Gene ID 12406
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-UBE2H antibody (Ubiquitin-Conjugating Enzyme E2H) (AA 1-184) (ABIN393686) Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-UBE2H antibody (Ubiquitin-Conjugating Enzyme E2H) (AA 1-184) (ABIN393686) anti-Ubiquitin-Conjugating Enzyme E2H (UBE2H) (AA 1-184) antibody (Image 2)
Background publications Lausen, Pless, Leonard, Kuvardina, Koch, Leutz: "Targets of the Tal1 transcription factor in erythrocytes: E2 ubiquitin conjugase regulation by Tal1." in: The Journal of biological chemistry, Vol. 285, Issue 8, pp. 5338-46, 2010 (PubMed).

Martin, Vourch, Mahé, Thépault, Antar, Védrine, Praline, Camu, Andres, Corcia,: "Association study of the ubiquitin conjugating enzyme gene UBE2H in sporadic ALS." in: Amyotrophic lateral sclerosis : official publication of the World Federation of Neurology Research Group on Motor Neuron Diseases, Vol. 10, Issue 5-6, pp. 432-5, 2009 (PubMed).

de Krom, Staal, Ophoff, Hendriks, Buitelaar, Franke, de Jonge, Bolton, Collier, Curran, van Engeland, van Ree: "A common variant in DRD3 receptor is associated with autism spectrum disorder." in: Biological psychiatry, Vol. 65, Issue 7, pp. 625-30, 2009 (PubMed).

Barbe, Lundberg, Oksvold, Stenius, Lewin, Björling, Asplund, Pontén, Brismar, Uhlén, Andersson-Svahn: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

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