Apoptosis Antagonizing Transcription Factor (AATF) antibody

Antigen: Apoptosis Antagonizing Transcription Factor (AATF)

Synonyms: DED, CHE1, CHE-1, Trb, Che-1, MGC35916, 4933415H02Rik, 5830465M17Rik, Ded, AATF, ded, che1, che-1, cb109, sb:cb109, wu:fc13d05, zgc:162071

Clonality: Monoclonal (3C7)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393689

Additional Information

Alternative name: AATF

Gene ID: 26574

Immunogen: AATF (AAH00591, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3C7

Description: Other names: CHE1, CHE1, DED apoptosis antagonizing transcription factor

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: AATF (AAH00591, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 63133 DA

Comments:

Background: The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to AATF (AT1003a) on HeLa cell . [antibody concentration 10 ug/ml] AATF monoclonal antibody (M04), clone 3C7 Western Blot analysis of AATF expression in Hela NE ( Cat # L013V3 ).

anti-Apoptosis Antagonizing Transcription Factor (AATF) antibody (Image 2)

Publications

Product: Kaul: "Cellular AATF gene: armour against HIV-1." in: Indian journal of biochemistry & biophysics, Vol. 44, Issue 5, pp. 276-8, 2008 (PubMed).

Wheeler, Metter, Tanaka et al.: "Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging." in: PLoS genetics, Vol. 5, Issue 10, pp. e1000685, 2009 (PubMed).

Haanpää, Reiman, Nikkilä et al.: "Mutation analysis of the AATF gene in breast cancer families." in: BMC cancer, Vol. 9, pp. 457, 2010 (PubMed).

Naukkarinen, Surakka, Pietiläinen et al.: "Use of genome-wide expression data to mine the "Gray Zone" of GWA studies leads to novel candidate obesity genes." in: PLoS genetics, Vol. 6, Issue 6, pp. e1000976, 2010 (PubMed).

Bruno, Desantis, Bossi et al.: "Che-1 promotes tumor cell survival by sustaining mutant p53 transcription and inhibiting DNA damage response activation." in: Cancer cell, Vol. 18, Issue 2, pp. 122-34, 2010 (PubMed).