LIM Domain Binding 3 (LDB3) antibody

Antigen: LIM Domain Binding 3 (LDB3)

Synonyms: ZASP, CYPHER, ORACLE, PDLIM6, ldb3z1, ldb3z4, FLJ35865, KIAA0613, KIAA01613, AW742271, MGC118603, ldb3l, zgc:55814, wu:fi31d08, ldb3, MGC52706, MGC75668, LDB3, RGD1564875

Clonality: Monoclonal (3C8)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393691

Additional Information

Alternative name: LDB3

Gene ID: 11155

Immunogen: LDB3 (AAH10929, 1 a.a. ~ 284 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 3C8

Description: Other names: CYPHER, KIAA01613, KIAA0613, ORACLE, PDLIM6, ZASP LIM domain binding 3

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: LDB3 (AAH10929, 1 a.a. ~ 284 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: NONE?! DA

Comments:

Background: This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified, all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of LDB3 over-expressed 293 cell line, cotransfected with LDB3 Validated Chimera RNAi ( Cat # H00011155-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with LDB3 monoclonal antibody (M06), clone 3C8 (Cat # AT2686a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of LDB3 expression in transfected 293T cell line by LDB3 monoclonal antibody (M06), clone 3C8. Lane 1: LDB3 transfected lysate(31 KDa). Lane 2: Non-transfected lysate.

anti-LIM Domain Binding 3 (LDB3) antibody (Image 2)

Publications

Product: Arimura, Inagaki, Hayashi et al.: "Impaired binding of ZASP/Cypher with phosphoglucomutase 1 is associated with dilated cardiomyopathy." in: Cardiovascular research, Vol. 83, Issue 1, pp. 80-8, 2009 (PubMed).

Aurino, Piluso, Saccone et al.: "Candidate-gene testing for orphan limb-girdle muscular dystrophies." in: Acta myologica : myopathies and cardiomyopathies : official journal of the Mediterranean Society of Myology / edited by the Gaetano Conte Academy for the study of striated muscle diseases, Vol. 27, pp. 90-7, 2009 (PubMed).

Vihola, Bachinski, Sirito et al.: "Differences in aberrant expression and splicing of sarcomeric proteins in the myotonic dystrophies DM1 and DM2." in: Acta neuropathologica, Vol. 119, Issue 4, pp. 465-79, 2010 (PubMed).

Rampersaud, Kinnamon, Hamilton et al.: "Common susceptibility variants examined for association with dilated cardiomyopathy." in: Annals of human genetics, Vol. 74, Issue 2, pp. 110-6, 2010 (PubMed).

Zimmerman, Cox, Lakdawala et al.: "A novel custom resequencing array for dilated cardiomyopathy." in: Genetics in medicine : official journal of the American College of Medical Genetics, Vol. 12, Issue 5, pp. 268-78, 2010 (PubMed).