LIM Domain Binding 3 (LDB3) antibody

Details for Product No. ABIN393692
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Antigen
Synonyms CMD1C, CYPHER, LDB3Z1, LDB3Z4, LVNC3, MFM4, ORACLE, PDLIM6, ZASP, AW742271, RGD1564875, ldb3l, zgc:55814, wu:fi31d08, ldb3, MGC52706, MGC75668, LDB3
Reactivity
Human
(38), (17), (16), (2), (2), (1)
Host
Mouse
(28), (6), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(30), (18), (10), (6), (3), (3), (2), (1)
Pubmed 5 references available
Catalog no. ABIN393692
Quantity 200 µL
Price
450.00 $   Plus shipping costs $45.00
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  • +1 404 474 4654
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Immunogen LDB3 (AAH10929, 1 a.a. ~ 284 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3C8
Isotype IgM kappa
Specificity LDB3 (AAH10929, 1 a.a. ~ 284 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification Purified
Alternative Name LDB3
Background This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified, all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains.
Synonyms: CYPHER, KIAA01613, KIAA0613, ORACLE, PDLIM6, ZASP LIM domain binding 3
Molecular Weight 77135 DA
Gene ID 11155
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified, all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains.

Restrictions For Research Use only
Format Liquid
Buffer Phosphate buffered saline, pH 7.2
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-LIM Domain Binding 3 (LDB3) antibody LDB3 monoclonal antibody (M06A), clone 3C8 Western Blot analysis of LDB3 expression in A-431. Detection limit for recombinant GST tagged LDB3 is approximately 0.03 ng/mL as a capture antibody.
anti-LIM Domain Binding 3 (LDB3) antibody (2) anti-LIM Domain Binding 3 (LDB3) antibody (Image 2)
Background publications Arimura, Inagaki, Hayashi et al.: "Impaired binding of ZASP/Cypher with phosphoglucomutase 1 is associated with dilated cardiomyopathy." in: Cardiovascular research, Vol. 83, Issue 1, pp. 80-8, 2009 (PubMed).

Aurino, Piluso, Saccone et al.: "Candidate-gene testing for orphan limb-girdle muscular dystrophies." in: Acta myologica : myopathies and cardiomyopathies : official journal of the Mediterranean Society of Myology / edited by the Gaetano Conte Academy for the study of striated muscle diseases, Vol. 27, pp. 90-7, 2009 (PubMed).

Vihola, Bachinski, Sirito et al.: "Differences in aberrant expression and splicing of sarcomeric proteins in the myotonic dystrophies DM1 and DM2." in: Acta neuropathologica, Vol. 119, Issue 4, pp. 465-79, 2010 (PubMed).

Rampersaud, Kinnamon, Hamilton et al.: "Common susceptibility variants examined for association with dilated cardiomyopathy." in: Annals of human genetics, Vol. 74, Issue 2, pp. 110-6, 2010 (PubMed).

Zimmerman, Cox, Lakdawala et al.: "A novel custom resequencing array for dilated cardiomyopathy." in: Genetics in medicine : official journal of the American College of Medical Genetics, Vol. 12, Issue 5, pp. 268-78, 2010 (PubMed).

Hosts (28), (6), (6)
Reactivities (38), (17), (16), (2), (2), (1)
Applications (30), (18), (10), (6), (3), (3), (2), (1)
Conjugates (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (7), (4), (4), (2), (2), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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