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Ring Finger Protein 139 (RNF139) (AA 565-665) antibody

Details for Product No. ABIN393698, Supplier: Log in to see
Antigen
  • 4930555P18Rik
  • DKFZp469C1333
  • HRCA1
  • RCA1
  • RNF139
  • TRC8
Epitope
AA 565-665
15
8
5
3
3
1
Reactivity
Human
50
19
18
4
1
1
1
1
1
Host
Mouse
23
17
12
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
39
30
14
11
11
1
Supplier
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Immunogen RNF139 (NP_009149, 565 a.a. ~ 665 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D10
Isotype IgG1 kappa
Specificity RNF139 (NP_009149, 565 a.a. ~ 665 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RNF139 (RNF139 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 75994 DA
Gene ID 12406
NCBI Accession NP_009149
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure, Cancer
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Ring Finger Protein 139 (RNF139) (AA 565-665) antibody (ABIN393698) RNF139 monoclonal antibody (M01), clone 3D10 Western Blot analysis of RNF139 expressi...
Immunohistochemistry (IHC) image for anti-Ring Finger Protein 139 (RNF139) (AA 565-665) antibody (ABIN393698) anti-Ring Finger Protein 139 (RNF139) (AA 565-665) antibody (Image 2)
Background publications Lee, Brauweiler, Rudolph, Hooper, Drabkin, Gemmill: "The TRC8 ubiquitin ligase is sterol regulated and interacts with lipid and protein biosynthetic pathways." in: Molecular cancer research : MCR, Vol. 8, Issue 1, pp. 93-106, 2010

Stagg, Thomas, van den Boomen, Wiertz, Drabkin, Gemmill, Lehner: "The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER." in: The Journal of cell biology, Vol. 186, Issue 5, pp. 685-92, 2009

Irisawa, Inoue, Ozawa, Mori, Sato et al.: "The sterol-sensing endoplasmic reticulum (ER) membrane protein TRC8 hampers ER to Golgi transport of sterol regulatory element-binding protein-2 (SREBP-2)/SREBP cleavage-activated protein and reduces ..." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28995-9004, 2009

Poland, Azim, Folsom, Goldfarb, Naeem, Korch, Drabkin, Gemmill, Plon: "A constitutional balanced t(3;8)(p14;q24.1) translocation results in disruption of the TRC8 gene and predisposition to clear cell renal cell carcinoma." in: Genes, chromosomes & cancer, Vol. 46, Issue 9, pp. 805-12, 2007

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006