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Details for Product No. ABIN393698

Ring Finger Protein 139 (RNF139) antibody

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Antigen
Synonyms HRCA1, RCA1, TRC8, 4930555P18Rik, RNF139, DKFZp469C1333
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN393698
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen RNF139 (NP_009149, 565 a.a. ~ 665 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D10
Isotype IgG1 kappa
Specificity RNF139 (NP_009149, 565 a.a. ~ 665 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RNF139
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 75994 DA
Gene ID 12406
UniProt NP_009149
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure, Cancer
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Poland, Azim, Folsom et al.: "A constitutional balanced t(3;8)(p14;q24.1) translocation results in disruption of the TRC8 gene and predisposition to clear cell renal cell carcinoma." in: Genes, chromosomes & cancer, Vol. 46, Issue 9, pp. 805-12, 2007 (PubMed).

Irisawa, Inoue, Ozawa et al.: "The sterol-sensing endoplasmic reticulum (ER) membrane protein TRC8 hampers ER to Golgi transport of sterol regulatory element-binding protein-2 (SREBP-2)/SREBP cleavage-activated protein and reduces SREBP-2 cleavage." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28995-9004, 2009 (PubMed).

Stagg, Thomas, van den Boomen et al.: "The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER." in: The Journal of cell biology, Vol. 186, Issue 5, pp. 685-92, 2009 (PubMed).

Lee, Brauweiler, Rudolph et al.: "The TRC8 ubiquitin ligase is sterol regulated and interacts with lipid and protein biosynthetic pathways." in: Molecular cancer research : MCR, Vol. 8, Issue 1, pp. 93-106, 2010 (PubMed).

Alternatives for antigen "Ring Finger Protein 139 (RNF139)", type "Antibodies"
Hosts (16), (7), (4)
Reactivities (23), (15), (13), (13), (13), (12), (12), (1)
Applications (17), (14), (10), (8), (2)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (3), (2), (1)
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