Ring Finger Protein 139 (RNF139) antibody

Antigen: Ring Finger Protein 139 (RNF139)

Synonyms: RCA1, TRC8, HRCA1, MGC31961, RNF139, DKFZp469C1333

Clonality: Monoclonal (3D10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393698

Additional Information

Alternative name: RNF139

Gene ID: 11236

UniProt: NP_009149

Immunogen: RNF139 (NP_009149, 565 a.a. ~ 665 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3D10

Description: Other names: HRCA1, MGC31961, RCA1, TRC8 ring finger protein 139

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: RNF139 (NP_009149, 565 a.a. ~ 665 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 75994 DA

Comments:

Background: The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Structure, Cancer

Restrictions: For Research Use only

Images

RNF139 monoclonal antibody (M01), clone 3D10 Western Blot analysis of RNF139 expression in HepG2 ( Cat # L019V1 ). Immunoperoxidase of monoclonal antibody to RNF139 ( Cat # AT3665a ) on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

anti-Ring Finger Protein 139 (RNF139) antibody (Image 2)

Publications

Product: Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Poland, Azim, Folsom et al.: "A constitutional balanced t(3;8)(p14;q24.1) translocation results in disruption of the TRC8 gene and predisposition to clear cell renal cell carcinoma." in: Genes, chromosomes & cancer, Vol. 46, Issue 9, pp. 805-12, 2007 (PubMed).

Irisawa, Inoue, Ozawa et al.: "The sterol-sensing endoplasmic reticulum (ER) membrane protein TRC8 hampers ER to Golgi transport of sterol regulatory element-binding protein-2 (SREBP-2)/SREBP cleavage-activated protein and reduces SREBP-2 cleavage." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28995-9004, 2009 (PubMed).

Stagg, Thomas, van den Boomen et al.: "The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER." in: The Journal of cell biology, Vol. 186, Issue 5, pp. 685-92, 2009 (PubMed).

Lee, Brauweiler, Rudolph et al.: "The TRC8 ubiquitin ligase is sterol regulated and interacts with lipid and protein biosynthetic pathways." in: Molecular cancer research : MCR, Vol. 8, Issue 1, pp. 93-106, 2010 (PubMed).