Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) antibody

Details for Product No. ABIN393701
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Antigen
Synonyms DDIT1, GADD45, Ddit1, Gadd45, AA545191, MGC53491, gadd45a, zgc:91795, GADD45A, ddit1, gadd45
Reactivity
Human
(38), (18), (17), (12), (12), (12)
Host
Mouse
(34), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(26), (10), (10), (4), (4), (4), (2), (1), (1)
Pubmed 5 references available
Catalog no. ABIN393701
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen GADD45A (AAH11757, 76 a.a. ~ 165 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D12
Isotype IgG1 kappa
Specificity GADD45A (AAH11757, 76 a.a. ~ 165 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name GADD45A
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 18336 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Yu, Di, Li et al.: "Genetic contribution of GADD45A to susceptibility to sporadic and non-BRCA1/2 familial breast cancers: a systematic evaluation in Chinese populations." in: Breast cancer research and treatment, Vol. 121, Issue 1, pp. 157-67, 2010 (PubMed).

Young, Hopkins, Hay et al.: "A gene-based risk score for lung cancer susceptibility in smokers and ex-smokers." in: Postgraduate medical journal, Vol. 85, Issue 1008, pp. 515-24, 2009 (PubMed).

Li, Zhao, Yin et al.: "Overexpression of the growth arrest and DNA damage-induced 45alpha gene contributes to autoimmunity by promoting DNA demethylation in lupus T cells." in: Arthritis and rheumatism, Vol. 62, Issue 5, pp. 1438-47, 2010 (PubMed).

van Roon, van Puijenbroek, Middeldorp et al.: "Early onset MSI-H colon cancer with MLH1 promoter methylation, is there a genetic predisposition?" in: BMC cancer, Vol. 10, pp. 180, 2010 (PubMed).

Sánchez, Pantoja-Uceda, Prieto et al.: "Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase." in: The Journal of biological chemistry, Vol. 285, Issue 29, pp. 22196-201, 2010 (PubMed).

Hosts (34), (4)
Reactivities (38), (18), (17), (12), (12), (12)
Applications (26), (10), (10), (4), (4), (4), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (3), (2), (2), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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